Laser-Mediated antibacterial effects of Few- and Multi-Layer Ti3C2Tx MXenes

Andreas Rosenkranz, Giordano Perini, Jose Y. Aguilar-Hurtado, Dario F. Zambrano, Bo Wang, Benedetta Niccolini, Patricia C. Henriques, Enrico Rosa, Flavio De Maio, Giovanni Delogu, Marco De Spirito, Valentina Palmieri, Massimiliano Papi

Research output: Contribution to journalArticlepeer-review

Abstract

Ti3C2TX nano-sheets (MXenes) with excellent light-conversion capacity have gained importance in treating infectious diseases due to their limited bacterial resistance. In this study, we exploit this property to design photothermal antibacterial therapy using few- (FX) and multi-layer (MX) Ti3C2Tx nano-sheets. We demonstrate that FX have a higher cytocompatibility and conversion of light to heat, but MX show a better efficacy in inhibiting growth of S. aureus and E. coli due to MXenes’ reversible bacteria trapping. For MX (25 µg/mL), □37% of E. coli and □23% of S. aureus cells survived, while the effect was less pronounced for FX with □72% of E. coli and □46% of S. aureus viable cells after treatment. After using 100 µg/mL of MX, □11% of E. coli and □4% of S. aureus survived, while FX had only a mild effect on both species. The NIR laser treatment increased the efficacy of both materials: 100 µg/mL of MX combined with 5 min laser treatment at 5.7 W cm − 2 completely killed both species. For FX, the treatment with 3 W cm − 2 and the highest concentration (100 µg/mL) induced an effect comparable to MX (87% on E. coli, 95% on S.aureus). The combined NIR-MXene treatment causes an irreversible cell death linked to the loss of cell integrity (DNA release quantification and bacteria debris observation).

Original languageEnglish
Article number150795
JournalApplied Surface Science
Volume567
DOIs
Publication statusPublished - Nov 30 2021

Keywords

  • Antibacterial
  • MXenes
  • Nanomaterials
  • Near infra-red
  • Photothermal therapy

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Physics and Astronomy(all)
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

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