Abstract
Pyramidal neurons in layers 2/3 and 5 of primary somatosensory cortex (S1) exhibit somewhat modest synaptic plasticity after whisker input deprivation. Whether neurons involved at earlier steps of sensory processing show more or less plasticity has not yet been examined. Here, we used longitudinal in vivo two-photon microscopy to investigate dendritic spine dynamics in apical tufts of GFP-expressing layer 4 (L4) pyramidal neurons of the vibrissal (barrel) S1 after unilateral whisker trimming. First, we characterize the molecular, anatomical, and electrophysiological properties of identified L4 neurons in Ebf2-Cre transgenic mice. Next, we show that input deprivation results in a substantial (∼50%) increase in the rate of dendritic spine loss, acutely (4–8 d) after whisker trimming. This robust synaptic plasticity in L4 suggests that primary thalamic recipient pyramidal neurons in S1 may be particularly sensitive to changes in sensory experience. Ebf2-Cre mice thus provide a useful tool for future assessment of initial steps of sensory processing in S1.
| Original language | English |
|---|---|
| Pages (from-to) | 7287-7294 |
| Number of pages | 8 |
| Journal | Journal of Neuroscience |
| Volume | 35 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - May 6 2015 |
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Keywords
- Barrel cortex
- Ebf2
- Electrophysiology
- Optogenetics
- Two-photon
- Whisker
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Layer 4 pyramidal neurons exhibit robust dendritic spine plasticity in vivo after input deprivation. / Miquelajauregui, Amaya; Kribakaran, Sahana; Mostany, Ricardo; Badaloni, Aurora; Consalez, G. Giacomo; Portera-Cailliau, Carlos.
In: Journal of Neuroscience, Vol. 35, No. 18, 06.05.2015, p. 7287-7294.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Layer 4 pyramidal neurons exhibit robust dendritic spine plasticity in vivo after input deprivation
AU - Miquelajauregui, Amaya
AU - Kribakaran, Sahana
AU - Mostany, Ricardo
AU - Badaloni, Aurora
AU - Consalez, G. Giacomo
AU - Portera-Cailliau, Carlos
PY - 2015/5/6
Y1 - 2015/5/6
N2 - Pyramidal neurons in layers 2/3 and 5 of primary somatosensory cortex (S1) exhibit somewhat modest synaptic plasticity after whisker input deprivation. Whether neurons involved at earlier steps of sensory processing show more or less plasticity has not yet been examined. Here, we used longitudinal in vivo two-photon microscopy to investigate dendritic spine dynamics in apical tufts of GFP-expressing layer 4 (L4) pyramidal neurons of the vibrissal (barrel) S1 after unilateral whisker trimming. First, we characterize the molecular, anatomical, and electrophysiological properties of identified L4 neurons in Ebf2-Cre transgenic mice. Next, we show that input deprivation results in a substantial (∼50%) increase in the rate of dendritic spine loss, acutely (4–8 d) after whisker trimming. This robust synaptic plasticity in L4 suggests that primary thalamic recipient pyramidal neurons in S1 may be particularly sensitive to changes in sensory experience. Ebf2-Cre mice thus provide a useful tool for future assessment of initial steps of sensory processing in S1.
AB - Pyramidal neurons in layers 2/3 and 5 of primary somatosensory cortex (S1) exhibit somewhat modest synaptic plasticity after whisker input deprivation. Whether neurons involved at earlier steps of sensory processing show more or less plasticity has not yet been examined. Here, we used longitudinal in vivo two-photon microscopy to investigate dendritic spine dynamics in apical tufts of GFP-expressing layer 4 (L4) pyramidal neurons of the vibrissal (barrel) S1 after unilateral whisker trimming. First, we characterize the molecular, anatomical, and electrophysiological properties of identified L4 neurons in Ebf2-Cre transgenic mice. Next, we show that input deprivation results in a substantial (∼50%) increase in the rate of dendritic spine loss, acutely (4–8 d) after whisker trimming. This robust synaptic plasticity in L4 suggests that primary thalamic recipient pyramidal neurons in S1 may be particularly sensitive to changes in sensory experience. Ebf2-Cre mice thus provide a useful tool for future assessment of initial steps of sensory processing in S1.
KW - Barrel cortex
KW - Ebf2
KW - Electrophysiology
KW - Optogenetics
KW - Two-photon
KW - Whisker
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UR - http://www.scopus.com/inward/citedby.url?scp=84929347142&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.5215-14.2015
DO - 10.1523/JNEUROSCI.5215-14.2015
M3 - Article
C2 - 25948276
AN - SCOPUS:84929347142
VL - 35
SP - 7287
EP - 7294
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 18
ER -