Layer 4 pyramidal neurons exhibit robust dendritic spine plasticity in vivo after input deprivation

Amaya Miquelajauregui, Sahana Kribakaran, Ricardo Mostany, Aurora Badaloni, G. Giacomo Consalez, Carlos Portera-Cailliau

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pyramidal neurons in layers 2/3 and 5 of primary somatosensory cortex (S1) exhibit somewhat modest synaptic plasticity after whisker input deprivation. Whether neurons involved at earlier steps of sensory processing show more or less plasticity has not yet been examined. Here, we used longitudinal in vivo two-photon microscopy to investigate dendritic spine dynamics in apical tufts of GFP-expressing layer 4 (L4) pyramidal neurons of the vibrissal (barrel) S1 after unilateral whisker trimming. First, we characterize the molecular, anatomical, and electrophysiological properties of identified L4 neurons in Ebf2-Cre transgenic mice. Next, we show that input deprivation results in a substantial (∼50%) increase in the rate of dendritic spine loss, acutely (4–8 d) after whisker trimming. This robust synaptic plasticity in L4 suggests that primary thalamic recipient pyramidal neurons in S1 may be particularly sensitive to changes in sensory experience. Ebf2-Cre mice thus provide a useful tool for future assessment of initial steps of sensory processing in S1.

Original languageEnglish
Pages (from-to)7287-7294
Number of pages8
JournalJournal of Neuroscience
Volume35
Issue number18
DOIs
Publication statusPublished - May 6 2015

Fingerprint

Vibrissae
Dendritic Spines
Pyramidal Cells
Neuronal Plasticity
Neurons
Somatosensory Cortex
Photons
Transgenic Mice
Microscopy

Keywords

  • Barrel cortex
  • Ebf2
  • Electrophysiology
  • Optogenetics
  • Two-photon
  • Whisker

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Layer 4 pyramidal neurons exhibit robust dendritic spine plasticity in vivo after input deprivation. / Miquelajauregui, Amaya; Kribakaran, Sahana; Mostany, Ricardo; Badaloni, Aurora; Consalez, G. Giacomo; Portera-Cailliau, Carlos.

In: Journal of Neuroscience, Vol. 35, No. 18, 06.05.2015, p. 7287-7294.

Research output: Contribution to journalArticle

Miquelajauregui, Amaya ; Kribakaran, Sahana ; Mostany, Ricardo ; Badaloni, Aurora ; Consalez, G. Giacomo ; Portera-Cailliau, Carlos. / Layer 4 pyramidal neurons exhibit robust dendritic spine plasticity in vivo after input deprivation. In: Journal of Neuroscience. 2015 ; Vol. 35, No. 18. pp. 7287-7294.
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