Lead Discovery of Dual G-Quadruplex Stabilizers and Poly(ADP-ribose) Polymerases (PARPs) Inhibitors: A New Avenue in Anticancer Treatment

E. Salvati, L. Botta, J. Amato, F. S. Di Leva, P. Zizza, A. Gioiello, B. Pagano, G. Graziani, M. Tarsounas, A. Randazzo, E. Novellino, A. Biroccio, S. Cosconati

Research output: Contribution to journalArticle

Abstract

G-quadruplex stabilizers are an established opportunity in anticancer chemotherapy. To circumvent the antiproliferative effects of G4 ligands, cancer cells recruit PARP enzymes at telomeres. Herein, starting from the structural similarity of a potent G4 ligand previously discovered by our group and a congeneric PARP inhibitor, a library of derivatives was synthesized to discover the first dual G4/PARP ligand. We demonstrate that a properly decorated thieno[3,2-c]quinolin-4(5H)-one stabilizes the G4 fold in vitro and in cells, induces a DNA damage response localized to telomeres, inhibits PARylation in cells, and has an antiproliferative effect in BRCA2 deficient tumor cells.
Original languageEnglish
Pages (from-to)3626-3635
Number of pages10
JournalJournal of Medicinal Chemistry
Volume60
Issue number9
DOIs
Publication statusPublished - May 11 2017

Keywords

  • Antineoplastic Agents/chemistry/pharmacology
  • Cell Line, Tumor
  • Drug Discovery
  • Drug Screening Assays, Antitumor
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Antibody Technique
  • G-Quadruplexes
  • Humans
  • Poly(ADP-ribose) Polymerase Inhibitors/chemistry/pharmacology

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    Salvati, E., Botta, L., Amato, J., Leva, F. S. D., Zizza, P., Gioiello, A., Pagano, B., Graziani, G., Tarsounas, M., Randazzo, A., Novellino, E., Biroccio, A., & Cosconati, S. (2017). Lead Discovery of Dual G-Quadruplex Stabilizers and Poly(ADP-ribose) Polymerases (PARPs) Inhibitors: A New Avenue in Anticancer Treatment. Journal of Medicinal Chemistry, 60(9), 3626-3635. https://doi.org/10.1021/acs.jmedchem.6b01563 [doi]