Left ventricular remodeling after experimental myocardial cryoinjury in rats

Michele M. Ciulla, Roberta Paliotti, Stefano Ferrero, Paola Braidotti, Arturo Esposito, Umberto Gianelli, Giuseppe Busca, Ugo Cioffi, Gaetano Bulfamante, Fabio Magrini

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The standard coronary ligation, the most studied model of experimental myocardial infarction in rats, is limited by high mortality and produces unpredictable areas of necrosis. To standardize the location and size of the infarct and to elucidate the mechanisms of myocardial remodeling and its progression to heart failure, we studied the functional, structural, and ultrastructural changes of myocardial infarction produced by experimental myocardial cryoinjury. The cryoinjury was successful in 24 (80%) of 30 male adult CD rats. A subepicardial infarct was documented on echocardiograms, with an average size of about 21%. Macroscopic examination reflected closely the stamp of the instrument used, without transition zones to viable myocardium. Histological examination, during the acute setting, revealed an extensive area of coagulation necrosis and hemorrhage in the subepicardium. An inflammatory infiltrate was evident since the 7th hour, whereas the reparative phase started within the first week, with proliferation of fibroblasts, endothelial cells, and myocytes. From the 7th day, deposition of collagen fibers was reported with a reparative scar completed at the 30th day. Ultrastructural study revealed vascular capillary damage and irreversible alterations of the myocytes in the acute setting and confirmed the histological findings of the later phases. The damage was associated with a progressive left ventricular (LV) remodeling, including thinning of the infarcted area, hypertrophy of the noninfarcted myocardium, and significant LV dilation. This process started from the 60th day and progressed over the subsequent 120 days period; at 180 days, a significant increase in LV filling pressure, indicative of heart failure, was found. In conclusion, myocardial cryodamage, although different in respect to ischemic damage, causes a standardized injury reproducing the cellular patterns of coagulation necrosis, early microvascular reperfusion, hemorrhage, inflammation, reparation, and scarring observed in myocardial infarction with a late evolution toward heart failure. This model is therefore suitable to study myocardial repair after injury.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalJournal of Surgical Research
Volume116
Issue number1
DOIs
Publication statusPublished - Jan 2004

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Ventricular Remodeling
Necrosis
Heart Failure
Myocardial Infarction
Muscle Cells
Cicatrix
Myocardium
Hemorrhage
Wounds and Injuries
Ventricular Pressure
Hypertrophy
Reperfusion
Ligation
Blood Vessels
Dilatation
Theoretical Models
Collagen
Endothelial Cells
Fibroblasts
Inflammation

Keywords

  • Cryoinjury
  • Left ventricular remodeling
  • Myocardial infarction

ASJC Scopus subject areas

  • Surgery

Cite this

Left ventricular remodeling after experimental myocardial cryoinjury in rats. / Ciulla, Michele M.; Paliotti, Roberta; Ferrero, Stefano; Braidotti, Paola; Esposito, Arturo; Gianelli, Umberto; Busca, Giuseppe; Cioffi, Ugo; Bulfamante, Gaetano; Magrini, Fabio.

In: Journal of Surgical Research, Vol. 116, No. 1, 01.2004, p. 91-97.

Research output: Contribution to journalArticle

Ciulla, MM, Paliotti, R, Ferrero, S, Braidotti, P, Esposito, A, Gianelli, U, Busca, G, Cioffi, U, Bulfamante, G & Magrini, F 2004, 'Left ventricular remodeling after experimental myocardial cryoinjury in rats', Journal of Surgical Research, vol. 116, no. 1, pp. 91-97. https://doi.org/10.1016/j.jss.2003.08.238
Ciulla, Michele M. ; Paliotti, Roberta ; Ferrero, Stefano ; Braidotti, Paola ; Esposito, Arturo ; Gianelli, Umberto ; Busca, Giuseppe ; Cioffi, Ugo ; Bulfamante, Gaetano ; Magrini, Fabio. / Left ventricular remodeling after experimental myocardial cryoinjury in rats. In: Journal of Surgical Research. 2004 ; Vol. 116, No. 1. pp. 91-97.
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