Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species

Blendi Ura, Lorenzo Monasta, Giorgio Arrigoni, Danilo Licastro, Giovanni Di Lorenzo, Federico Romano, Bartolomea Gaita, Federica Scrimin, Giuseppe Ricci

Research output: Contribution to journalArticle

Abstract

Uterine leiomyomas are benign smooth muscle cell tumors originating from the myometrium. The present study focused on leiomyoma and myometrium phosphoproteome enrichment by using immobilized metal affinity chromatography (IMAC). The phosphoproteome was analyzed by two-dimensional gel electrophoresis coupled with mass spectrometry. Western blotting was used for data validation. The results from IMAC identified 26 proteins significantly differentially phosphorylated in leiomyomas compared with normal myometrium. Three upregulated proteins (peroxiredoxin 2, protein disulfide isomerase family A member 3 and peroxiredoxin 4) were further validated by western blotting. Ingenuity pathway analysis revealed that four phosphoproteins were involved in the inhibition of oxidative stress and synthesis of reactive oxygen species. The present results demonstated for the first time an association between oxidative stress and phosphorylation in leiomyoma development.

Original languageEnglish
Pages (from-to)2329-2335
Number of pages7
JournalInternational Journal of Molecular Medicine
Volume44
Issue number6
DOIs
Publication statusPublished - Jan 1 2019

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Keywords

  • Leiomyoma
  • Mass spectrometry
  • Myometrium
  • Oxidative stress
  • Phosphoproteomics
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Genetics

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