Length variation of glycoprotein 120 V2 region in relation to biological phenotypes and coreceptor usage of primary HIV type 1 isolates

M. Jansson, E. Backström, G. Scarlatti, Å Björndal, S. Matsuda, P. Rossi, J. Albert, H. Wigzell

Research output: Contribution to journalArticle

Abstract

Conflicting data have been published concerning the correlation between the length of the second variable region (V2) in the HIV-1 envelope and the biological phenotype of the virus. Here the V2 region length of primary HIV-1 isolates was compared with biological phenotype and coreceptor usage. The V2 region variation was determined by DNA fragment length analysis, virus biological phenotype by the MT-2 cell assay, and coreceptor usage by infection of U87.CD4 cells expressing CCR3, CCR5, or CXCR4. Ninety-three primary virus isolates from 40 patients were analyzed. This panel of viruses included sequential isolates obtained from patients who progressed to AIDS with or without a virus phenotypic switch. We found that NSI MT-2-negative isolates had significantly shorter V2 regions than SI MT-2-positive isolates. However, when V2 region lengths of viruses were analyzed in more detail, we observed that NSI isolates obtained from patients shortly before the phenotypic switch had V2 region lengths similar to those of SI isolates. V2 regions of NSI isolates obtained from patients who progressed to AIDS without a virus phenotypic switch had, in contrast, shorter V2 region than isolates obtained just before virus phenotypic switch. Coreceptor analysis revealed that CCR5-using (R5) isolates generally had shorter V2 regions than virus isolates with the ability to enter CXCR4-expressing cells. Moreover, no significant difference in V2 region length was observed between monotropic SI isolates, that is, X4 isolates, and multitropic SI isolates, that is, R3R5X4 or R5X4 isolates. Thus, we conclude that R5 NSI isolates obtained from patients with stable virus phenotype through the whole disease course display shorter V2 regions than isolates obtained from patients at switch of virus phenotype, suggesting that V2 region length may influence virus coreceptor usage.

Original languageEnglish
Pages (from-to)1405-1414
Number of pages10
JournalAIDS Research and Human Retroviruses
Volume17
Issue number15
DOIs
Publication statusPublished - 2001

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HIV-1
Glycoproteins
Viruses
Phenotype
Acquired Immunodeficiency Syndrome
DNA

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Length variation of glycoprotein 120 V2 region in relation to biological phenotypes and coreceptor usage of primary HIV type 1 isolates. / Jansson, M.; Backström, E.; Scarlatti, G.; Björndal, Å; Matsuda, S.; Rossi, P.; Albert, J.; Wigzell, H.

In: AIDS Research and Human Retroviruses, Vol. 17, No. 15, 2001, p. 1405-1414.

Research output: Contribution to journalArticle

Jansson, M. ; Backström, E. ; Scarlatti, G. ; Björndal, Å ; Matsuda, S. ; Rossi, P. ; Albert, J. ; Wigzell, H. / Length variation of glycoprotein 120 V2 region in relation to biological phenotypes and coreceptor usage of primary HIV type 1 isolates. In: AIDS Research and Human Retroviruses. 2001 ; Vol. 17, No. 15. pp. 1405-1414.
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