TY - JOUR
T1 - Lentiviral-mediated RNAi in vivo silencing of Col6a1, a gene with complex tissue specific expression pattern
AU - Frka, Kosjenka
AU - Facchinello, Nicola
AU - Del Vecchio, Claudia
AU - Carpi, Andrea
AU - Curtarello, Matteo
AU - Venerando, Rina
AU - Angelin, Alessia
AU - Parolin, Cristina
AU - Bernardi, Paolo
AU - Bonaldo, Paolo
AU - Volpin, Dino
AU - Braghetta, Paola
AU - Bressan, Giorgio M.
PY - 2009/4/20
Y1 - 2009/4/20
N2 - RNA interference (RNAi) through the use of lentiviral vectors is a valuable technique to induce loss of function mutations in mammals. Although very promising, the method has found only limited application and its general applicability remains to be established. Here we analyze how different factors influence RNAi mediated silencing of Col6a1, a gene of the extracellular matrix with a complex pattern of tissue specific expression. Our results, obtained with vectors pLVTHM and pLVPT-rtTRKRAB, point out three parameters as major determinants of the efficiency of interference: the choice of interfering sequence, the number of proviral copies integrated into the mouse genome and the site of insertion of the provirus. Although low copy number may produce efficient interference with low frequency, the general trend is that the number of integrated proviral copies determines the level of silencing and the severity of phenotypic traits. The site of insertion not only determines the overall intensity of expression of the small interfering RNA (siRNA), but also introduces slight variability of silencing in different organs. A lentiviral vector (pLVPT-rtTRKRAB) with doxycycline-inducible production of siRNA was also tested. Control of expression by the drug was stringent in many tissues; however, in some tissues turning off of siRNA synthesis was not complete. The data support the application of lentiviral vectors used here in transgenesis.
AB - RNA interference (RNAi) through the use of lentiviral vectors is a valuable technique to induce loss of function mutations in mammals. Although very promising, the method has found only limited application and its general applicability remains to be established. Here we analyze how different factors influence RNAi mediated silencing of Col6a1, a gene of the extracellular matrix with a complex pattern of tissue specific expression. Our results, obtained with vectors pLVTHM and pLVPT-rtTRKRAB, point out three parameters as major determinants of the efficiency of interference: the choice of interfering sequence, the number of proviral copies integrated into the mouse genome and the site of insertion of the provirus. Although low copy number may produce efficient interference with low frequency, the general trend is that the number of integrated proviral copies determines the level of silencing and the severity of phenotypic traits. The site of insertion not only determines the overall intensity of expression of the small interfering RNA (siRNA), but also introduces slight variability of silencing in different organs. A lentiviral vector (pLVPT-rtTRKRAB) with doxycycline-inducible production of siRNA was also tested. Control of expression by the drug was stringent in many tissues; however, in some tissues turning off of siRNA synthesis was not complete. The data support the application of lentiviral vectors used here in transgenesis.
KW - Collagen VI
KW - Lentiviral vectors
KW - RNA interference
KW - Transgenic mice
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U2 - 10.1016/j.jbiotec.2009.02.013
DO - 10.1016/j.jbiotec.2009.02.013
M3 - Article
C2 - 19428725
AN - SCOPUS:64249083170
VL - 141
SP - 8
EP - 17
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 1-2
ER -