Leptin activates Stat3, Stat1 and AP-1 in mouse adipose tissue

P. Bendinelli, P. Maroni, F. Pecori Giraldi, R. Piccoletti

Research output: Contribution to journalArticle

Abstract

Intraperitoneal leptin administration to wild-type and ob/ob mice caused a prompt activation of Stat1 and Stat3, the former to a lesser extent, in epididymal adipose tissue. Immunoblot experiments showed that tyrosine phosphorylation of Stat3 increased in total cellular extracts and that the phosphorylated protein translocated into the nucleus upon leptin treatment. Tyrosine phosphorylation and nuclear translocation of Stat1 were evident only in ob/ob mice. Gel shift and supershift analyses showed that leptin activated sis-inducible element (SIE) binding activity of adipose nuclear extracts, with Stat3 homodimer as the predominant complex. Stat1/3 heterodimers and Stat1 homodimers take part as well in the response in wild-type and ob/ob mice, although to a lesser degree. AP-1 binding activity was also induced in adipose tissue by in vivo leptin treatment with a time course that suggests a post-transcriptional inductive mechanism. This effect was greater in the ob/ob than in wild-type mice. Our data indicate that leptin operates in vivo directly on adipose tissue by triggering responses that modulate gene expression. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)11-20
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume168
Issue number1-2
DOIs
Publication statusPublished - Oct 25 2000

Keywords

  • AP-1
  • Leptin
  • Mouse white adipose tissue
  • STAT

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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