Leptin attenuates ischemia-reperfusion injury in the rat liver

Marco Carbone, Luisa Campagnolo, Mario Angelico, Giuseppe Tisone, Cristiana Almerighi, Claudia Telesca, Ilaria Lenci, Ilana Moscatelli, Renato Massoud, Leonardo Baiocchi

Research output: Contribution to journalArticle

Abstract

Leptin is an adipocytokine that reduces ischemic damage in several organs including brain and heart. STAT3 activation is a key step for the attainment of leptin effects in various tissues. We evaluated the possible effect of leptin on liver viability and STAT3 activation, in a rat model of ischemia-reperfusion injury. Rat livers, flushed and stored with Belzer solution (4° C for 24 h), were warmly reperfused (3.5 ml/min/g liver for 1 h at 37°C with O 2) with Krebs-Ringer bicarbonate. Treatment group underwent an identical protocol with the adjunct of Leptin (10 ng/ml). Liver effluent was harvested to assess LDH and AST output. Liver tissue was used for pSTAT3 expression (western blot and immunostaining), optical microscopy, TUNEL, and Cell Death Detection assays. The pSTAT3 expression was enhanced by administration of leptin. In parallel, LDH and AST output were reduced (P = 0.04 and P = 0.02 for LDH and AST, respectively). Optical microscopy, TUNEL, and Cell Death Detection assay results demonstrated increased viability in livers treated with leptin in comparison with others (Optical microscopy P = 0.02; TUNEL P = 0.01; Cell death Detection assay P = 0.003). In conclusion, cold storage and reperfusion with leptin reduce liver ischemia-reperfusion injury. This effect is associated with an increased expression of pSTAT-3.

Original languageEnglish
Pages (from-to)1282-1288
Number of pages7
JournalTransplant International
Volume25
Issue number12
DOIs
Publication statusPublished - Dec 2012

Keywords

  • ischemia-reperfusion injury
  • leptin
  • liver
  • rat
  • STAT3

ASJC Scopus subject areas

  • Transplantation

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    Carbone, M., Campagnolo, L., Angelico, M., Tisone, G., Almerighi, C., Telesca, C., Lenci, I., Moscatelli, I., Massoud, R., & Baiocchi, L. (2012). Leptin attenuates ischemia-reperfusion injury in the rat liver. Transplant International, 25(12), 1282-1288. https://doi.org/10.1111/j.1432-2277.2012.01555.x