Leptin-leptin receptor are involved in angiogenesis in human hepatocellular carcinoma

Domenico Ribatti, Anna Sandra Belloni, Beatrice Nico, Mariasevera Di Comite, Enrico Crivellato, Angelo Vacca

Research output: Contribution to journalArticle

Abstract

Recent evidence in vitro and in vivo indicates that leptin, an adipose tissue-secreted hormone which is involved in the regulation of satiety, metabolic rate and thermogenesis, is implicated in angiogenesis. However, the role of leptin-mediated angiogenesis in hepatic carcinogenesis has not yet been completely elucidated. In this study, we have correlated microvascular density and leptin/leptin receptor (Ob-R) expression in endothelial and tumor cells with the histopathological type in human hepatocellular carcinoma (HCC). For this purpose, specimens of 40 primary HCC were submitted to immunohistochemical investigation using anti-CD31, anti-leptin and anti-Ob-R antibodies. Poorly-differentiated HCC had a higher degree of vascularization than other stages and leptin/Ob-R expression in both tumor and endothelial cells increased in parallel with the grade of malignancy and was highly correlated with the degree of angiogenesis. In the chick embryo chorioallantoic membrane in vivo assay, HCC biopsy specimens induced a strong angiogenic response, which was counteracted by an anti-leptin antibody. Taken together, these findings indicate that leptin/Ob-R correlate with angiogenesis and tumor progression in patients with HCC and that an anti-leptin antibody exerts an angiostatic activity in HCC.

Original languageEnglish
Pages (from-to)1596-1602
Number of pages7
JournalPeptides
Volume29
Issue number9
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Leptin Receptors
Leptin
Hepatocellular Carcinoma
Tumors
Antibodies
Anti-Idiotypic Antibodies
Neoplasms
Endothelial Cells
Chorioallantoic Membrane
Thermogenesis
Biopsy
Endothelial cells
Chick Embryo
Adipose Tissue
Assays
Carcinogenesis
Cells
Hormones
Tissue
Membranes

Keywords

  • Angiogenesis
  • Antiangiogenesis
  • Chorioallantoic membrane
  • Hepatocellular carcinoma
  • Leptin
  • Leptin receptor

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Ribatti, D., Belloni, A. S., Nico, B., Di Comite, M., Crivellato, E., & Vacca, A. (2008). Leptin-leptin receptor are involved in angiogenesis in human hepatocellular carcinoma. Peptides, 29(9), 1596-1602. https://doi.org/10.1016/j.peptides.2008.05.011

Leptin-leptin receptor are involved in angiogenesis in human hepatocellular carcinoma. / Ribatti, Domenico; Belloni, Anna Sandra; Nico, Beatrice; Di Comite, Mariasevera; Crivellato, Enrico; Vacca, Angelo.

In: Peptides, Vol. 29, No. 9, 09.2008, p. 1596-1602.

Research output: Contribution to journalArticle

Ribatti, D, Belloni, AS, Nico, B, Di Comite, M, Crivellato, E & Vacca, A 2008, 'Leptin-leptin receptor are involved in angiogenesis in human hepatocellular carcinoma', Peptides, vol. 29, no. 9, pp. 1596-1602. https://doi.org/10.1016/j.peptides.2008.05.011
Ribatti D, Belloni AS, Nico B, Di Comite M, Crivellato E, Vacca A. Leptin-leptin receptor are involved in angiogenesis in human hepatocellular carcinoma. Peptides. 2008 Sep;29(9):1596-1602. https://doi.org/10.1016/j.peptides.2008.05.011
Ribatti, Domenico ; Belloni, Anna Sandra ; Nico, Beatrice ; Di Comite, Mariasevera ; Crivellato, Enrico ; Vacca, Angelo. / Leptin-leptin receptor are involved in angiogenesis in human hepatocellular carcinoma. In: Peptides. 2008 ; Vol. 29, No. 9. pp. 1596-1602.
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