Leucine-enkephalin increases the level of inositol (1,4,5) triphosphate and releases calcium from an intracellular pool in rat ventricular cardiac myocytes.

C. Ventura, E. G. Lakatta, A. Sisini, M. P. Campus, M. C. Capogrossi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In rat ventricular cardiomyocytes loaded with the fluorescent Ca2+ indicator Indo-1/AM, the delta opioid receptor agonist Leu-Enk caused Cai oscillations and abolished the caffeine-induced Cai transient. During superfusion of cardiomyocytes with the specific opioid antagonist naloxone, Cai is not affected by Leu-Enk and the caffeine-triggered Cai transient is preserved. In parallel experiments with cardiac myocytes, the delta opioid agonist increased the intracellular level of Ins (1,4,5) P3 by about 4 times above the control value. Such an effect was completely antagonized by naloxone. Thus, Leu-Enk induces depletion of Ca2+ from the SR by a receptor-mediated mechanism which appears to involve an increase in the intracellular level of Ins (1,4,5) P3.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalBollettino della Societa Italiana di Biologia Sperimentale
Volume67
Issue number3
Publication statusPublished - Mar 1991

Fingerprint

Leucine Enkephalin
Inositol 1,4,5-Trisphosphate
Cardiac Myocytes
Naloxone
Caffeine
Calcium
delta Opioid Receptor
Narcotic Antagonists
Opioid Analgesics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Leucine-enkephalin increases the level of inositol (1,4,5) triphosphate and releases calcium from an intracellular pool in rat ventricular cardiac myocytes. / Ventura, C.; Lakatta, E. G.; Sisini, A.; Campus, M. P.; Capogrossi, M. C.

In: Bollettino della Societa Italiana di Biologia Sperimentale, Vol. 67, No. 3, 03.1991, p. 261-266.

Research output: Contribution to journalArticle

@article{c3f10d9355ce4c7daf67f105b4fcd741,
title = "Leucine-enkephalin increases the level of inositol (1,4,5) triphosphate and releases calcium from an intracellular pool in rat ventricular cardiac myocytes.",
abstract = "In rat ventricular cardiomyocytes loaded with the fluorescent Ca2+ indicator Indo-1/AM, the delta opioid receptor agonist Leu-Enk caused Cai oscillations and abolished the caffeine-induced Cai transient. During superfusion of cardiomyocytes with the specific opioid antagonist naloxone, Cai is not affected by Leu-Enk and the caffeine-triggered Cai transient is preserved. In parallel experiments with cardiac myocytes, the delta opioid agonist increased the intracellular level of Ins (1,4,5) P3 by about 4 times above the control value. Such an effect was completely antagonized by naloxone. Thus, Leu-Enk induces depletion of Ca2+ from the SR by a receptor-mediated mechanism which appears to involve an increase in the intracellular level of Ins (1,4,5) P3.",
author = "C. Ventura and Lakatta, {E. G.} and A. Sisini and Campus, {M. P.} and Capogrossi, {M. C.}",
year = "1991",
month = "3",
language = "English",
volume = "67",
pages = "261--266",
journal = "Bollettino della Societa Italiana di Biologia Sperimentale",
issn = "0037-8771",
publisher = "Casa Editrice Idelson Gnocchi",
number = "3",

}

TY - JOUR

T1 - Leucine-enkephalin increases the level of inositol (1,4,5) triphosphate and releases calcium from an intracellular pool in rat ventricular cardiac myocytes.

AU - Ventura, C.

AU - Lakatta, E. G.

AU - Sisini, A.

AU - Campus, M. P.

AU - Capogrossi, M. C.

PY - 1991/3

Y1 - 1991/3

N2 - In rat ventricular cardiomyocytes loaded with the fluorescent Ca2+ indicator Indo-1/AM, the delta opioid receptor agonist Leu-Enk caused Cai oscillations and abolished the caffeine-induced Cai transient. During superfusion of cardiomyocytes with the specific opioid antagonist naloxone, Cai is not affected by Leu-Enk and the caffeine-triggered Cai transient is preserved. In parallel experiments with cardiac myocytes, the delta opioid agonist increased the intracellular level of Ins (1,4,5) P3 by about 4 times above the control value. Such an effect was completely antagonized by naloxone. Thus, Leu-Enk induces depletion of Ca2+ from the SR by a receptor-mediated mechanism which appears to involve an increase in the intracellular level of Ins (1,4,5) P3.

AB - In rat ventricular cardiomyocytes loaded with the fluorescent Ca2+ indicator Indo-1/AM, the delta opioid receptor agonist Leu-Enk caused Cai oscillations and abolished the caffeine-induced Cai transient. During superfusion of cardiomyocytes with the specific opioid antagonist naloxone, Cai is not affected by Leu-Enk and the caffeine-triggered Cai transient is preserved. In parallel experiments with cardiac myocytes, the delta opioid agonist increased the intracellular level of Ins (1,4,5) P3 by about 4 times above the control value. Such an effect was completely antagonized by naloxone. Thus, Leu-Enk induces depletion of Ca2+ from the SR by a receptor-mediated mechanism which appears to involve an increase in the intracellular level of Ins (1,4,5) P3.

UR - http://www.scopus.com/inward/record.url?scp=0026131090&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026131090&partnerID=8YFLogxK

M3 - Article

C2 - 1657046

AN - SCOPUS:0026131090

VL - 67

SP - 261

EP - 266

JO - Bollettino della Societa Italiana di Biologia Sperimentale

JF - Bollettino della Societa Italiana di Biologia Sperimentale

SN - 0037-8771

IS - 3

ER -