Leucine-rich repeat kinase 2 phosphorylation on synapsin I regulates glutamate release at pre-synaptic sites

Antonella Marte, Isabella Russo, Claudia Rebosio, Pierluigi Valente, Elisa Belluzzi, Francesca Pischedda, Caterina Montani, Chiara Lavarello, Andrea Petretto, Ernesto Fedele, Pietro Baldelli, Fabio Benfenati, Giovanni Piccoli, Elisa Greggio, Franco Onofri

Research output: Contribution to journalArticle

Abstract

Leucine-rich repeat kinase 2 (LRRK2) is a large multidomain scaffolding protein with kinase and GTPase activities involved in synaptic vesicle (SV) dynamics. While its role in Parkinson’s disease has been largely investigated, little is known about LRRK2 physiological role and until now few proteins have been described as substrates. We have previously demonstrated that LRRK2 through its WD40 domain interacts with synapsin I, an important SV-associated phosphoprotein involved in neuronal development and in the regulation of neurotransmitter release. To test whether synapsin I is substrate for LRRK2 and characterize the properties of its phosphorylation, we used in vitro kinase and binding assays as well as cellular model and site-direct mutagenesis. Using synaptosomes in superfusion, patch-clamp recordings in autaptic WT and synapsin I KO cortical neurons and SypHy assay on primary cortical culture from wild-type and BAC human LRRK2 G2019S mice we characterized the role of LRRK2 kinase activity on glutamate release and SV trafficking. Here we reported that synapsin I is phosphorylated by LRRK2 and demonstrated that the interaction between LRRK2 WD40 domain and synapsin I is crucial for this phosphorylation. Moreover, we showed that LRRK2 phosphorylation of synapsin I at threonine 337 and 339 significantly reduces synapsin I-SV/actin interactions. Using complementary experimental approaches, we demonstrated that LRRK2 controls glutamate release and SV dynamics in a kinase activity and synapsin I-dependent manner. Our findings show that synapsin I is a LRRK2 substrate and describe a novel mechanisms of regulation of glutamate release by LRRK2 kinase activity. (Figure presented.).

Original languageEnglish
Pages (from-to)264-281
Number of pages18
JournalJournal of Neurochemistry
Volume150
Issue number3
DOIs
Publication statusPublished - Aug 2019

Fingerprint

Synapsins
Phosphorylation
Leucine
Glutamic Acid
Phosphotransferases
Synaptic Vesicles
Assays
Substrates
Synaptosomes
Mutagenesis
GTP Phosphohydrolases
Phosphoproteins

Keywords

  • leucine-rich repeat kinase 2
  • neurotransmitter release
  • Parkinson’s disease
  • phosphorylation
  • synapsin I

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Leucine-rich repeat kinase 2 phosphorylation on synapsin I regulates glutamate release at pre-synaptic sites. / Marte, Antonella; Russo, Isabella; Rebosio, Claudia; Valente, Pierluigi; Belluzzi, Elisa; Pischedda, Francesca; Montani, Caterina; Lavarello, Chiara; Petretto, Andrea; Fedele, Ernesto; Baldelli, Pietro; Benfenati, Fabio; Piccoli, Giovanni; Greggio, Elisa; Onofri, Franco.

In: Journal of Neurochemistry, Vol. 150, No. 3, 08.2019, p. 264-281.

Research output: Contribution to journalArticle

Marte, Antonella ; Russo, Isabella ; Rebosio, Claudia ; Valente, Pierluigi ; Belluzzi, Elisa ; Pischedda, Francesca ; Montani, Caterina ; Lavarello, Chiara ; Petretto, Andrea ; Fedele, Ernesto ; Baldelli, Pietro ; Benfenati, Fabio ; Piccoli, Giovanni ; Greggio, Elisa ; Onofri, Franco. / Leucine-rich repeat kinase 2 phosphorylation on synapsin I regulates glutamate release at pre-synaptic sites. In: Journal of Neurochemistry. 2019 ; Vol. 150, No. 3. pp. 264-281.
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AU - Marte, Antonella

AU - Russo, Isabella

AU - Rebosio, Claudia

AU - Valente, Pierluigi

AU - Belluzzi, Elisa

AU - Pischedda, Francesca

AU - Montani, Caterina

AU - Lavarello, Chiara

AU - Petretto, Andrea

AU - Fedele, Ernesto

AU - Baldelli, Pietro

AU - Benfenati, Fabio

AU - Piccoli, Giovanni

AU - Greggio, Elisa

AU - Onofri, Franco

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N2 - Leucine-rich repeat kinase 2 (LRRK2) is a large multidomain scaffolding protein with kinase and GTPase activities involved in synaptic vesicle (SV) dynamics. While its role in Parkinson’s disease has been largely investigated, little is known about LRRK2 physiological role and until now few proteins have been described as substrates. We have previously demonstrated that LRRK2 through its WD40 domain interacts with synapsin I, an important SV-associated phosphoprotein involved in neuronal development and in the regulation of neurotransmitter release. To test whether synapsin I is substrate for LRRK2 and characterize the properties of its phosphorylation, we used in vitro kinase and binding assays as well as cellular model and site-direct mutagenesis. Using synaptosomes in superfusion, patch-clamp recordings in autaptic WT and synapsin I KO cortical neurons and SypHy assay on primary cortical culture from wild-type and BAC human LRRK2 G2019S mice we characterized the role of LRRK2 kinase activity on glutamate release and SV trafficking. Here we reported that synapsin I is phosphorylated by LRRK2 and demonstrated that the interaction between LRRK2 WD40 domain and synapsin I is crucial for this phosphorylation. Moreover, we showed that LRRK2 phosphorylation of synapsin I at threonine 337 and 339 significantly reduces synapsin I-SV/actin interactions. Using complementary experimental approaches, we demonstrated that LRRK2 controls glutamate release and SV dynamics in a kinase activity and synapsin I-dependent manner. Our findings show that synapsin I is a LRRK2 substrate and describe a novel mechanisms of regulation of glutamate release by LRRK2 kinase activity. (Figure presented.).

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