Leucocytes telomere length and breast cancer risk/ susceptibility: A case-control study

Research output: Contribution to journalArticle

Abstract

Background Telomere length in peripheral blood leukocytes (PBL-TL) was proposed as a biomarker of cancer risk. Recent scientific evidence suggested PBL-TL plays a diverse role in different cancers. Inconsistent results were obtained on PBL-TL in relation to breast cancer risk and specifically to the presence of BRCA1 and BRCA2 mutations. The aim of the present case-control study was to analyse the correlation between family history of breast cancer or presence of a BRCA mutation and PBL-TL in the hypothesis that TL is a modifier of cancer risk. Methods PBL-TL was measured using the real-time quantitative PCR method in DNA for 142 cases and 239 controls. All the women enrolled were characterized for cancer family history. A subgroup of 48 women were classified for the presence of a BRCA mutation. PBL-TL were summarized as means and standard deviations, and compared by standard analysis of variance. A multivariable Generalised Linear Model was fitted to the data with PBL-TL as the dependent variable, case/control status and presence of a BRCA/VUS mutation as factors, and age in 4 strata as a covariate. Results Age was significantly associated with decreasing PBL-TL in controls (p = 0.01), but not in BC cases. The telomere length is shorter in cases than in controls after adjusting for age. No effect on PBL-TL of BMI, smoke nor of the most common risk factors for breast cancer was observed. No association between PBL-TL and family history was detected both in BC cases and controls. In the multivariate model, no association was observed between BRCA mutation and decreased PBL-TL. A statistically significant interaction (p = 0.031) between case-control status and a BRCA-mutation/VUS was observed, but no effect was detected for the interaction of cancer status and BRCA or VUS.

Original languageEnglish
Article numbere0197522
JournalPLoS One
Volume13
Issue number5
DOIs
Publication statusPublished - May 1 2018

Fingerprint

telomeres
Telomere
case-control studies
breast neoplasms
Case-Control Studies
leukocytes
Leukocytes
Breast Neoplasms
mutation
Mutation
neoplasms
Neoplasms
modifiers (genes)
Age Factors
smoke
Tumor Biomarkers
Smoke
Analysis of variance (ANOVA)
Real-Time Polymerase Chain Reaction
Linear Models

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Leucocytes telomere length and breast cancer risk/ susceptibility : A case-control study. / Pavanello, Sofia; Varesco, Liliana; Gismondi, Viviana; Bruzzi, Paolo; Bolognesi, Claudia.

In: PLoS One, Vol. 13, No. 5, e0197522, 01.05.2018.

Research output: Contribution to journalArticle

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