Leukocyte recruitment in the cerebrospinal fluid of mice with experimental meningitis is inhibited by an antibody to junctional adhesion molecule (JAM)

Aldo Del Maschio, Ada De Luigi, Ines Martin-Padura, Manfred Brockhaus, Tamas Bartfai, Paolo Fruscella, Luciano Adorini, Gian Vito Martino, Roberto Furlan, Maria Grazia De Simoni, Elisabetta Dejana

Research output: Contribution to journalArticle

Abstract

The mechanisms that govern leukocyte transmigration through the endothelium are not yet fully defined. Junctional adhesion molecule (JAM) is a newly cloned member of the immunoglobulin superfamily which is selectively concentrated at tight junctions of endothelial and epithelial cells. A blocking monoclonal antibody (BV11 mAb) directed to JAM was able to inhibit monocyte transmigration through endothelial cells in in vitro and in vivo chemotaxis assays. In this study, we report that BV11 administration was able to attenuate cytokine-induced meningitis in mice. The intravenous injection of BV11 mAb significantly inhibited leukocyte accumulation in the cerebrospinal fluid and infiltration in the brain parenchyma. Blood-brain barrier permeability was also reduced by the mAb. We conclude that JAM may be a new target in limiting the inflammatory response that accompanies meningitis.

Original languageEnglish
Pages (from-to)1351-1356
Number of pages6
JournalJournal of Experimental Medicine
Volume190
Issue number9
DOIs
Publication statusPublished - Nov 1 1999

Keywords

  • Blood-brain barrier
  • Endothelium
  • Meningitis
  • Tight junction
  • Vascular permeability

ASJC Scopus subject areas

  • Immunology

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