Leukocyte telomere length in Alzheimer's disease patients with a different rate of progression

Enzo Tedone, Beatrice Arosio, Federico Colombo, Evelyn Ferri, Delphine Asselineau, Francois Piette, Cristina Gussago, Joel Belmin, Sylvie Pariel, Khadija Benlhassan, Martina Casati, Anne Bornand, Paolo Dionigi Rossi, Paolo Mazzola, Giorgio Annoni, Mohamed Doulazmi, Jean Mariani, Laura Porretti, Dorothy H. Bray, Daniela Mari

Research output: Contribution to journalArticle

Abstract

Background: Age and short leukocyte telomeres have been associated with a higher risk of Alzheimer's disease (AD). Inflammation is involved in AD and it is suggested that anti-inflammatory interleukin-10 (IL-10) may partly antagonize these processes. Objective: The aim is to correlate telomere length (TL) in peripheral blood mononuclear cells (PBMC) from patients with AD to disease progression rate. Moreover, we evaluated whether TL was associated with IL-10 production by unstimulated or amyloid-β (Aβ)-stimulated PBMC. Methods: We enrolled 31 late-onset AD and 20 age-matched healthy elderly (HE). After a two-year follow-up period, patients were retrospectively evaluated as slow-progressing (ADS) (Mini Mental State Examination (MMSE) decline over the two years of follow-up ≤3 points) or fast progressing AD (ADF) (MMSE decline ≥5 points). TL was measured by flow cytometry and in vitro IL-10 production by enzyme-linked immunosorbent assay. Results: TL (mean±SD) for HE, ADS, and ADFwas 2.3±0.1, 2.0±0.1, and 2.5±0.1 Kb, respectively. ADS showed a shorter TL compared to HE (p = 0.034) and to ADF (p = 0.005). MMSE decline correlated with TL in AD (R2 = 0.284; p = 0.008). We found a significant difference in IL-10 production between unstimulated and Aβ-stimulated PBMC from ADS (40.7±13.7 versus 59.0±27.0; p = 0.004) but not from ADF (39.7±14.4 versus 42.2±22.4). HE showed a trend toward significance (47.1±25.4 versus 55.3±27.9; p = 0.10). Conclusion: PBMC from ADF may be characterized by an impaired response induced by Aβ and by a reduced proliferative response responsible for the longer telomeres. TL might be a contributing factor in predicting the rate of AD progression.

Original languageEnglish
Pages (from-to)761-769
Number of pages9
JournalJournal of Alzheimer's Disease
Volume46
Issue number3
DOIs
Publication statusPublished - Jun 25 2015

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Telomere
Alzheimer Disease
Leukocytes
Interleukin-10
Blood Cells
Disease Progression
Amyloid
Flow Cytometry
Anti-Inflammatory Agents
Enzyme-Linked Immunosorbent Assay
Inflammation

Keywords

  • Alzheimer's disease
  • disease progression
  • interleukin-10
  • peripheral blood mononuclear cells
  • telomere

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Geriatrics and Gerontology
  • Clinical Psychology

Cite this

Leukocyte telomere length in Alzheimer's disease patients with a different rate of progression. / Tedone, Enzo; Arosio, Beatrice; Colombo, Federico; Ferri, Evelyn; Asselineau, Delphine; Piette, Francois; Gussago, Cristina; Belmin, Joel; Pariel, Sylvie; Benlhassan, Khadija; Casati, Martina; Bornand, Anne; Rossi, Paolo Dionigi; Mazzola, Paolo; Annoni, Giorgio; Doulazmi, Mohamed; Mariani, Jean; Porretti, Laura; Bray, Dorothy H.; Mari, Daniela.

In: Journal of Alzheimer's Disease, Vol. 46, No. 3, 25.06.2015, p. 761-769.

Research output: Contribution to journalArticle

Tedone, E, Arosio, B, Colombo, F, Ferri, E, Asselineau, D, Piette, F, Gussago, C, Belmin, J, Pariel, S, Benlhassan, K, Casati, M, Bornand, A, Rossi, PD, Mazzola, P, Annoni, G, Doulazmi, M, Mariani, J, Porretti, L, Bray, DH & Mari, D 2015, 'Leukocyte telomere length in Alzheimer's disease patients with a different rate of progression', Journal of Alzheimer's Disease, vol. 46, no. 3, pp. 761-769. https://doi.org/10.3233/JAD-142808
Tedone, Enzo ; Arosio, Beatrice ; Colombo, Federico ; Ferri, Evelyn ; Asselineau, Delphine ; Piette, Francois ; Gussago, Cristina ; Belmin, Joel ; Pariel, Sylvie ; Benlhassan, Khadija ; Casati, Martina ; Bornand, Anne ; Rossi, Paolo Dionigi ; Mazzola, Paolo ; Annoni, Giorgio ; Doulazmi, Mohamed ; Mariani, Jean ; Porretti, Laura ; Bray, Dorothy H. ; Mari, Daniela. / Leukocyte telomere length in Alzheimer's disease patients with a different rate of progression. In: Journal of Alzheimer's Disease. 2015 ; Vol. 46, No. 3. pp. 761-769.
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AU - Tedone, Enzo

AU - Arosio, Beatrice

AU - Colombo, Federico

AU - Ferri, Evelyn

AU - Asselineau, Delphine

AU - Piette, Francois

AU - Gussago, Cristina

AU - Belmin, Joel

AU - Pariel, Sylvie

AU - Benlhassan, Khadija

AU - Casati, Martina

AU - Bornand, Anne

AU - Rossi, Paolo Dionigi

AU - Mazzola, Paolo

AU - Annoni, Giorgio

AU - Doulazmi, Mohamed

AU - Mariani, Jean

AU - Porretti, Laura

AU - Bray, Dorothy H.

AU - Mari, Daniela

PY - 2015/6/25

Y1 - 2015/6/25

N2 - Background: Age and short leukocyte telomeres have been associated with a higher risk of Alzheimer's disease (AD). Inflammation is involved in AD and it is suggested that anti-inflammatory interleukin-10 (IL-10) may partly antagonize these processes. Objective: The aim is to correlate telomere length (TL) in peripheral blood mononuclear cells (PBMC) from patients with AD to disease progression rate. Moreover, we evaluated whether TL was associated with IL-10 production by unstimulated or amyloid-β (Aβ)-stimulated PBMC. Methods: We enrolled 31 late-onset AD and 20 age-matched healthy elderly (HE). After a two-year follow-up period, patients were retrospectively evaluated as slow-progressing (ADS) (Mini Mental State Examination (MMSE) decline over the two years of follow-up ≤3 points) or fast progressing AD (ADF) (MMSE decline ≥5 points). TL was measured by flow cytometry and in vitro IL-10 production by enzyme-linked immunosorbent assay. Results: TL (mean±SD) for HE, ADS, and ADFwas 2.3±0.1, 2.0±0.1, and 2.5±0.1 Kb, respectively. ADS showed a shorter TL compared to HE (p = 0.034) and to ADF (p = 0.005). MMSE decline correlated with TL in AD (R2 = 0.284; p = 0.008). We found a significant difference in IL-10 production between unstimulated and Aβ-stimulated PBMC from ADS (40.7±13.7 versus 59.0±27.0; p = 0.004) but not from ADF (39.7±14.4 versus 42.2±22.4). HE showed a trend toward significance (47.1±25.4 versus 55.3±27.9; p = 0.10). Conclusion: PBMC from ADF may be characterized by an impaired response induced by Aβ and by a reduced proliferative response responsible for the longer telomeres. TL might be a contributing factor in predicting the rate of AD progression.

AB - Background: Age and short leukocyte telomeres have been associated with a higher risk of Alzheimer's disease (AD). Inflammation is involved in AD and it is suggested that anti-inflammatory interleukin-10 (IL-10) may partly antagonize these processes. Objective: The aim is to correlate telomere length (TL) in peripheral blood mononuclear cells (PBMC) from patients with AD to disease progression rate. Moreover, we evaluated whether TL was associated with IL-10 production by unstimulated or amyloid-β (Aβ)-stimulated PBMC. Methods: We enrolled 31 late-onset AD and 20 age-matched healthy elderly (HE). After a two-year follow-up period, patients were retrospectively evaluated as slow-progressing (ADS) (Mini Mental State Examination (MMSE) decline over the two years of follow-up ≤3 points) or fast progressing AD (ADF) (MMSE decline ≥5 points). TL was measured by flow cytometry and in vitro IL-10 production by enzyme-linked immunosorbent assay. Results: TL (mean±SD) for HE, ADS, and ADFwas 2.3±0.1, 2.0±0.1, and 2.5±0.1 Kb, respectively. ADS showed a shorter TL compared to HE (p = 0.034) and to ADF (p = 0.005). MMSE decline correlated with TL in AD (R2 = 0.284; p = 0.008). We found a significant difference in IL-10 production between unstimulated and Aβ-stimulated PBMC from ADS (40.7±13.7 versus 59.0±27.0; p = 0.004) but not from ADF (39.7±14.4 versus 42.2±22.4). HE showed a trend toward significance (47.1±25.4 versus 55.3±27.9; p = 0.10). Conclusion: PBMC from ADF may be characterized by an impaired response induced by Aβ and by a reduced proliferative response responsible for the longer telomeres. TL might be a contributing factor in predicting the rate of AD progression.

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