Abstract
Objective: We performed a cross-sectional study to examine the differences in leukocyte telomere length among three groups of subjects: patients with type 2 diabetes mellitus without history of previous myocardial infarction (Type2DM), patients with type 2 diabetes mellitus with evidence of previous myocardial infarction (Type2DM + MI), and healthy control subjects (CTR). The main objective of the present study is to investigate differences in telomere length between the studied groups of subjects, with the aim to clarify if telomere length could be a reliable marker associated with MI in Type2DM patients. Secondary end point is the identification of associations between leukocyte telomere length and selected variables related to glycemic control, pro-inflammatory status and lipidic profile. Research design and methods: A total of 272 elderly subjects, 103 Type2DM (mean age 70 ± 4 years, 59% males), 65 Type2DM + MI (mean age 68 ± 7 years, 68% males), and 104 CTR (mean age 69 ± 7 years, 50% males) were studied. Telomere length, defined as T/S (Telomere-Single copy gene ratio), was determined in leukocytes by quantitative real-time polymerase chain reaction (real-time PCR)-based assay. Moreover, we assessed: (1) high sensitive C reactive protein (hsCRP), fibrinogen and plasminogen-activator inibitor-1 (PAI-1) as inflammatory markers; (2) fasting glucose, insulin, glycated haemoglobin (HbA1C) and waist-to-hip ratio as markers of glycemic control; (3) total-cholesterol, HDL-cholesterol and triglycerides as markers of lipidic profile, in all sample population. The use of statins and sulfonylurea, as well as the presence of some relevant diabetes complications (nephropathy and retinopathy) were also assessed. Conclusion: Type2DM + MI elderly patients have leukocyte telomere lengths shorter than those of Type2DM (without MI) and healthy CTR. Moreover, glucose, HbA1C and waist-to-hip ratio, variables related to glycemic control, showed a significant inverse correlation with leukocyte telomeres length.
| Original language | English |
|---|---|
| Pages (from-to) | 588-593 |
| Number of pages | 6 |
| Journal | Atherosclerosis |
| Volume | 206 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 2009 |
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Keywords
- Aging
- Myocardial infarction
- Telomere length
- Type2 diabetes mellitus
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Leukocyte telomere shortening in elderly Type2DM patients with previous myocardial infarction. / Olivieri, Fabiola; Lorenzi, Maria; Antonicelli, Roberto; Testa, Roberto; Sirolla, Cristina; Cardelli, Maurizio; Mariotti, Serena; Marchegiani, Francesca; Marra, Maurizio; Spazzafumo, Liana; Bonfigli, Anna Rita; Procopio, Antonio.
In: Atherosclerosis, Vol. 206, No. 2, 10.2009, p. 588-593.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Leukocyte telomere shortening in elderly Type2DM patients with previous myocardial infarction
AU - Olivieri, Fabiola
AU - Lorenzi, Maria
AU - Antonicelli, Roberto
AU - Testa, Roberto
AU - Sirolla, Cristina
AU - Cardelli, Maurizio
AU - Mariotti, Serena
AU - Marchegiani, Francesca
AU - Marra, Maurizio
AU - Spazzafumo, Liana
AU - Bonfigli, Anna Rita
AU - Procopio, Antonio
PY - 2009/10
Y1 - 2009/10
N2 - Objective: We performed a cross-sectional study to examine the differences in leukocyte telomere length among three groups of subjects: patients with type 2 diabetes mellitus without history of previous myocardial infarction (Type2DM), patients with type 2 diabetes mellitus with evidence of previous myocardial infarction (Type2DM + MI), and healthy control subjects (CTR). The main objective of the present study is to investigate differences in telomere length between the studied groups of subjects, with the aim to clarify if telomere length could be a reliable marker associated with MI in Type2DM patients. Secondary end point is the identification of associations between leukocyte telomere length and selected variables related to glycemic control, pro-inflammatory status and lipidic profile. Research design and methods: A total of 272 elderly subjects, 103 Type2DM (mean age 70 ± 4 years, 59% males), 65 Type2DM + MI (mean age 68 ± 7 years, 68% males), and 104 CTR (mean age 69 ± 7 years, 50% males) were studied. Telomere length, defined as T/S (Telomere-Single copy gene ratio), was determined in leukocytes by quantitative real-time polymerase chain reaction (real-time PCR)-based assay. Moreover, we assessed: (1) high sensitive C reactive protein (hsCRP), fibrinogen and plasminogen-activator inibitor-1 (PAI-1) as inflammatory markers; (2) fasting glucose, insulin, glycated haemoglobin (HbA1C) and waist-to-hip ratio as markers of glycemic control; (3) total-cholesterol, HDL-cholesterol and triglycerides as markers of lipidic profile, in all sample population. The use of statins and sulfonylurea, as well as the presence of some relevant diabetes complications (nephropathy and retinopathy) were also assessed. Conclusion: Type2DM + MI elderly patients have leukocyte telomere lengths shorter than those of Type2DM (without MI) and healthy CTR. Moreover, glucose, HbA1C and waist-to-hip ratio, variables related to glycemic control, showed a significant inverse correlation with leukocyte telomeres length.
AB - Objective: We performed a cross-sectional study to examine the differences in leukocyte telomere length among three groups of subjects: patients with type 2 diabetes mellitus without history of previous myocardial infarction (Type2DM), patients with type 2 diabetes mellitus with evidence of previous myocardial infarction (Type2DM + MI), and healthy control subjects (CTR). The main objective of the present study is to investigate differences in telomere length between the studied groups of subjects, with the aim to clarify if telomere length could be a reliable marker associated with MI in Type2DM patients. Secondary end point is the identification of associations between leukocyte telomere length and selected variables related to glycemic control, pro-inflammatory status and lipidic profile. Research design and methods: A total of 272 elderly subjects, 103 Type2DM (mean age 70 ± 4 years, 59% males), 65 Type2DM + MI (mean age 68 ± 7 years, 68% males), and 104 CTR (mean age 69 ± 7 years, 50% males) were studied. Telomere length, defined as T/S (Telomere-Single copy gene ratio), was determined in leukocytes by quantitative real-time polymerase chain reaction (real-time PCR)-based assay. Moreover, we assessed: (1) high sensitive C reactive protein (hsCRP), fibrinogen and plasminogen-activator inibitor-1 (PAI-1) as inflammatory markers; (2) fasting glucose, insulin, glycated haemoglobin (HbA1C) and waist-to-hip ratio as markers of glycemic control; (3) total-cholesterol, HDL-cholesterol and triglycerides as markers of lipidic profile, in all sample population. The use of statins and sulfonylurea, as well as the presence of some relevant diabetes complications (nephropathy and retinopathy) were also assessed. Conclusion: Type2DM + MI elderly patients have leukocyte telomere lengths shorter than those of Type2DM (without MI) and healthy CTR. Moreover, glucose, HbA1C and waist-to-hip ratio, variables related to glycemic control, showed a significant inverse correlation with leukocyte telomeres length.
KW - Aging
KW - Myocardial infarction
KW - Telomere length
KW - Type2 diabetes mellitus
UR - http://www.scopus.com/inward/record.url?scp=70349432260&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349432260&partnerID=8YFLogxK
U2 - 10.1016/j.atherosclerosis.2009.03.034
DO - 10.1016/j.atherosclerosis.2009.03.034
M3 - Article
C2 - 19464008
AN - SCOPUS:70349432260
VL - 206
SP - 588
EP - 593
JO - Atherosclerosis
JF - Atherosclerosis
SN - 0021-9150
IS - 2
ER -