Levels of human erythrocyte membrane-bound and cytosolic glycohydrolases are associated with oxidative stress in erectile dysfunction patients

L. Massaccesi, G. V. Melzi D'Eril, G. M. Colpi, G. Tettamanti, G. Goi, A. Barassi

Research output: Contribution to journalArticle

Abstract

Oxidative stress (OS) and production of NO, by endothelium nitric oxide synthetase (eNOS), are involved in the pathophysiology of erectile dysfunction (ED). Moreover, OS induces modifications of the physicochemical properties of erythrocyte (RBC) plasma membranes and of the enzyme content of the same membranes. Due to their role in signalling early membrane alterations in OSrelated pathologies, several plasmamembrane and cytosolic glycohydrolases of human RBC have been proposed as newmarkers of cellular OS. In RBC, NOS can be activated and deactivated by phosphorylation/glycosylation. In this regulatorymechanism O-β-N-AcetylGlucosaminidase is a key enzyme. Cellular levels of O-GlcNAcylated proteins are related to OS; consequently dysfunctional eNOS O-GlcNAcylation seems to have a crucial role in ED. To elucidate the possible association between RBC glycohydrolases and OS, plasma hydroperoxides and antioxidant total defenses (Lag-time), cytosolic O-β-N-AcetylGlucosaminidase, cytosolic and membrane Hexosaminidase, membrane β-D-Glucuronidase, and α-D-Glucosidase have been studied in 39 ED patients and 30 controls. In ED subjects hydroperoxides and plasma membrane glycohydrolases activities are significantly increased whereas Lagtime values and cytosolic glycohydrolases activities are significantly decreased. These data confirm the strong OS status in ED patients, the role of the studied glycohydrolases as early OS biomarker and suggest their possible use as specific marker of ED patients, particularly in those undergoing nutritional/pharmacological antioxidant therapy.

Original languageEnglish
Article number485917
JournalDisease Markers
Volume2014
DOIs
Publication statusPublished - 2014

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ASJC Scopus subject areas

  • Biochemistry, medical
  • Clinical Biochemistry
  • Molecular Biology
  • Genetics

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