Levels of pre-S antigens and HBV DNA in sera from high and low viremic HBV carriers

A. R. Garbuglia, A. Manzin, A. Budkowska, G. Taliani, M. Clementi, C. Delfini, G. Carloni

Research output: Contribution to journalArticle

Abstract

The biological and clinical significances of pre-S antigens and HBV replication were investigated. Some 125 sera, 28 from HBeAg and 97 from anti-HBe-positive HBsAg, carriers were studied. The aim was to verify whether pre-S antigens could be expressed in serum in complete absence of viremia. Pre-S proteins, determined by an enzyme immunoassay, were found in sera regardless of the presence of HBV DNA, as detected by spot-hybridization. The sera without detectable HBV DNA were investigated further by PCR using specific primers for the S and C regions of HBV. PCR analysis of samples revealed that 4 out of 5 HBeAg and 33 out of 41 (80.5%) anti-HBe positive sera contained HBV-amplified sequences of S and C regions. Pre-S antigen values correlated well with the amounts of HBV DNA in serum detected by PCR in anti-HBe-positive subjects with high titers of pre-S antigens (104-106). In addition, PCR highlighted the presence of HBV DNA sequences in 8 out of 17 (47.1%) pre-S-negative HBsAg-positive sera.

Original languageEnglish
Pages (from-to)273-282
Number of pages10
JournalJournal of Medical Virology
Volume35
Issue number4
Publication statusPublished - 1991

Keywords

  • Chronic carriers
  • HBe-status
  • HBV viremia
  • PCR

ASJC Scopus subject areas

  • Virology

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    Garbuglia, A. R., Manzin, A., Budkowska, A., Taliani, G., Clementi, M., Delfini, C., & Carloni, G. (1991). Levels of pre-S antigens and HBV DNA in sera from high and low viremic HBV carriers. Journal of Medical Virology, 35(4), 273-282.