Levetiracetam does not modulate neuronal voltage-gated Na+ and T-type Ca2+ currents

C. Zona, I. Niespodziany, C. Marchetti, H. Klitgaard, G. Bernardi, D. G. Margineanu

Research output: Contribution to journalArticle

Abstract

This study investigated whether the mechanism of action of levetiracetam (LEV) is related to effects on neuronal voltage-gated Na+ or T-type Ca2+ currents. Rat neocortical neurones in culture were subjected to the whole-cell mode of voltage clamping under experimental conditions designed to study voltage-gated Na+ current. Additionally, visually identified pyramidal neurones in the CA1 area of rat hippocampal slices were subjected to the whole-cell mode of voltage clamping under experimental conditions designed to study low-voltage-gated (T-type) Ca2+ current. LEV (10 μM-1 mM) did not modify the Na+ current amplitude and did not change (200 μM) the steady-state activation and inactivation, the time to peak, the fast kinetics of the inactivation and the recovery from the steady-state inactivation of the Na+ current. Likewise, LEV (32-100 μM) did not modify the amplitude and did not change the steady-state activation and inactivation, the time to peak, the fast kinetics of the inactivation and the recovery from the steady-state inactivation of the T-type Ca2+ current. In conclusion, neuronal voltage-gated Na+ channels do not appear directly involved in the antiepileptic mechanism of action of LEV, and LEV was devoid of effect on the low-voltage-gated (T-type) Ca2+ current in hippocampal neurones.

Original languageEnglish
Pages (from-to)279-286
Number of pages8
JournalSeizure
Volume10
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • Antiepileptic drugs
  • Levetiracetam
  • Low-voltage-gated calcium current
  • Rat
  • Sodium current

ASJC Scopus subject areas

  • Clinical Neurology
  • Pediatrics, Perinatology, and Child Health
  • Neurology
  • Psychology(all)

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  • Cite this

    Zona, C., Niespodziany, I., Marchetti, C., Klitgaard, H., Bernardi, G., & Margineanu, D. G. (2001). Levetiracetam does not modulate neuronal voltage-gated Na+ and T-type Ca2+ currents. Seizure, 10(4), 279-286. https://doi.org/10.1053/seiz.2000.0504