Ligand and voltage gated sodium channels may regulate electrogenic pump activity in human, mouse and rat lymphocytes

Carlo Pieri, Rina Recchioni, Fausto Moroni, László Balkay, Teréz Márián, Lajos Trón, Sándor Damjanovich

Research output: Contribution to journalArticle

Abstract

Bretylium tosylate - a sodium channel opener - resulted in an increase of membrane potential of depolarized human, rat and mouse T and B lymphocytes. Flow cytometric membrane potential measurements with bis-oxonol revealed that the above hyperpolarizing effect was amiloride, ouabain, tetrodotoxin, azide and temperature sensitive. The effect showed an absolute dependence on the extracellular sodium but it was insensitive to the extracellular Ca2+ level. The voltage gating of the effect can be eliminated by either an increase of the extracellular potassium concentration or low doses of veratrin. The existence of a voltage and ligand gated sodium channel is suggested in the plasma membrane of all kinds of lymphocytes. The hyperpolarization is explained by an increased activity of the electrogenic sodium-potassium ATP-ase. Induced opening of such sodium channels may regulate the electrogenic pump activity and indirectly cell activation.

Original languageEnglish
Pages (from-to)999-1002
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume160
Issue number3
DOIs
Publication statusPublished - May 15 1989

Fingerprint

Voltage-Gated Sodium Channels
Lymphocytes
Sodium Channels
Human Activities
Membrane Potentials
Rats
Potassium
Bretylium Tosylate
Sodium
Pumps
Ligands
Ligand-Gated Ion Channels
Azides
Amiloride
Tetrodotoxin
Ouabain
Membranes
B-Lymphocytes
Adenosine Triphosphate
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ligand and voltage gated sodium channels may regulate electrogenic pump activity in human, mouse and rat lymphocytes. / Pieri, Carlo; Recchioni, Rina; Moroni, Fausto; Balkay, László; Márián, Teréz; Trón, Lajos; Damjanovich, Sándor.

In: Biochemical and Biophysical Research Communications, Vol. 160, No. 3, 15.05.1989, p. 999-1002.

Research output: Contribution to journalArticle

Pieri, Carlo ; Recchioni, Rina ; Moroni, Fausto ; Balkay, László ; Márián, Teréz ; Trón, Lajos ; Damjanovich, Sándor. / Ligand and voltage gated sodium channels may regulate electrogenic pump activity in human, mouse and rat lymphocytes. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 160, No. 3. pp. 999-1002.
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AU - Márián, Teréz

AU - Trón, Lajos

AU - Damjanovich, Sándor

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