Ligase detection reaction and universal array as a tool to detect grapevine infecting phytoplasmas

Andrea Frosini, P. Casati, P. A. Bianco, R. Bordoni, C. Consolandi, B. Castiglioni, A. Mezzelani, E. Rizzi, C. Battaglia, G. Belli, L. Rossi Bernardi, G. De Bellis

Research output: Contribution to journalArticle

Abstract

Phytoplasmas are unculturable wall less bacteria involved in many plant diseases; in particular Flavescence dorée represents a severe threat to viticulture, because of its epidemic behavior in field. Here we present the application of the LDR technique, associated with hybridisation on universal arrays, as a quick and reliable tool for plant quarantine procedures and mandatory measures, in order to distinguish between different phytoplasmas' induced infections, compared to classical protocols in use, particularly PCR - RFLP assays.

Original languageEnglish
Pages (from-to)265-267
Number of pages3
JournalMinerva Biotecnologica
Volume14
Issue number3-4
Publication statusPublished - Dec 2002

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Keywords

  • Flavescence dorée
  • Gene expression profiling
  • Gene expression regulation
  • Oligonucleotide array sequence
  • Plant disease

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Frosini, A., Casati, P., Bianco, P. A., Bordoni, R., Consolandi, C., Castiglioni, B., Mezzelani, A., Rizzi, E., Battaglia, C., Belli, G., Rossi Bernardi, L., & De Bellis, G. (2002). Ligase detection reaction and universal array as a tool to detect grapevine infecting phytoplasmas. Minerva Biotecnologica, 14(3-4), 265-267.