Light-induced fluorescence spectroscopy of adenomas, adenocarcinomas and non-neoplastic mucosa in human colon I. In vitro measurements

Renato Marchesini, Marco Brambilla, Emanuele Pignoli, Giovanni Bottiroli, Anna Cleta Croce, Marco Dal Fante, Pasquale Spinelli, Silvana di Palma

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

In an attempt to evaluate whether induced fluorescence could be exploited to discriminate neoplastic from non-neoplastic tissue, fluorescence spectroscopy was performed at 450-800 nm on 83 biopsy specimens of colonic mucosa. Measurements showed that fluorescence spectra of adenoma, adenocarcinoma and non-neoplastic mucosa manifest dissimilar patterns. Nine variables, whose photophysical and/or biological bases need further investigation, were derived from the spectra. Discriminant functions between the groups of lesions were determined by using a stepwise discriminant analysis. The diagnostic test had a sensitivity of 80.6% and 88.2%, and a specificity of 90.5% and 95.2% in discriminating neoplastic from non-neoplastic mucosa and adenoma from non-neoplastic mucosa respectively. These results suggest that fluorescence spectroscopy has the potential to improve endoscopic diagnosis of premalignant and malignant lesions of colonic mucosa.

Original languageEnglish
Pages (from-to)219-230
Number of pages12
JournalJournal of Photochemistry and Photobiology B: Biology
Volume14
Issue number3
DOIs
Publication statusPublished - Jul 15 1992

Fingerprint

fluorescence emission spectroscopy
adenoma
Fluorescence Spectrometry
Fluorescence spectroscopy
adenocarcinoma
Adenoma
colon
mucosa
Colon
Mucous Membrane
Adenocarcinoma
Light
fluorescence
lesions
spectroscopy
lesions (animal)
Fluorescence
Biopsy
Discriminant Analysis
Discriminant analysis

Keywords

  • adenocarcinoma
  • adenoma
  • human colon
  • Native fluorescence
  • spectrofluorimeter.

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Light-induced fluorescence spectroscopy of adenomas, adenocarcinomas and non-neoplastic mucosa in human colon I. In vitro measurements. / Marchesini, Renato; Brambilla, Marco; Pignoli, Emanuele; Bottiroli, Giovanni; Croce, Anna Cleta; Dal Fante, Marco; Spinelli, Pasquale; di Palma, Silvana.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 14, No. 3, 15.07.1992, p. 219-230.

Research output: Contribution to journalArticle

Marchesini, Renato ; Brambilla, Marco ; Pignoli, Emanuele ; Bottiroli, Giovanni ; Croce, Anna Cleta ; Dal Fante, Marco ; Spinelli, Pasquale ; di Palma, Silvana. / Light-induced fluorescence spectroscopy of adenomas, adenocarcinomas and non-neoplastic mucosa in human colon I. In vitro measurements. In: Journal of Photochemistry and Photobiology B: Biology. 1992 ; Vol. 14, No. 3. pp. 219-230.
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AU - Croce, Anna Cleta

AU - Dal Fante, Marco

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