Light microscopic identification and immunocytochemical characterization of glutamatergic synapses in brain sections

Marcello Melone, Alain Burette, Richard J. Weinberg

Research output: Contribution to journalArticle

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Abstract

Presynaptic proteins are readily identified by light microscopic immunocytochemistry, but immunodetection of postsynaptic proteins in brain sections proves difficult. We performed immunofluorescent double labeling for the NR1 subunit of the N-methyl-D-aspartate receptor (NMDAR) and the vesicular glutamate transporter 1 (VGLUT1). In material fixed with 4% paraformaldehyde, NMDAR staining in somatosensory cortex was restricted to the section surface, whereas presynaptic staining extended deeper into the tissue. Staining for postsynaptic proteins was enhanced in weakly fixed material and in tissue treated with pepsin, as previously reported, but tissue quality was impaired. Staining was also markedly enhanced, and without impairment of tissue quality, by treatment during perfusion with a mixture of inhibitors of proteases and the ubiquitin/proteosome system. We performed quantitative analysis of confocal images to study how immunostaining varies with depth into the tissue. Virtually all puncta immunopositive for VGLUT1 colocalized with synaptophysin puncta; these presynaptic puncta were most numerous 1-2 μm the section surface. In contrast, puncta immunopositive for the NR1 subunit were most numerous at the surface, as were puncta immunopositive for the NR2 subunit, SynGAP, and CaMKII. Punctate staining for all postsynaptic proteins, but not presynaptic markers, was substantially enhanced in material pretreated with antiproteolytic agents. The large majority of NR1-positive puncta at the surface associated with VGLUT1 in this material are likely to represent synaptic contacts. Approximately eighty-five percent of VGLUT1-positive puncta in layers II-III of SI are associated with NR1-positive puncta, and ∼80% are associated with NR2, SynGAP, and CaMKII. This approach may permit systematic analysis of the chemistry of glutamatergic synapses with light microscopic immunocytochemistry.

Original languageEnglish
Pages (from-to)495-509
Number of pages15
JournalJournal of Comparative Neurology
Volume492
Issue number4
DOIs
Publication statusPublished - Nov 28 2005

Fingerprint

Vesicular Glutamate Transport Protein 1
Synapses
Staining and Labeling
Light
Brain
Calcium-Calmodulin-Dependent Protein Kinase Type 2
N-Methyl-D-Aspartate Receptors
Proteins
Immunohistochemistry
Synaptophysin
Somatosensory Cortex
Pepsin A
Ubiquitin
Protease Inhibitors
Perfusion

Keywords

  • Confocal microscopy
  • NMDA receptor
  • Postsynaptic proteins
  • Presynaptic proteins
  • SynGAP
  • Ubiquitin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Light microscopic identification and immunocytochemical characterization of glutamatergic synapses in brain sections. / Melone, Marcello; Burette, Alain; Weinberg, Richard J.

In: Journal of Comparative Neurology, Vol. 492, No. 4, 28.11.2005, p. 495-509.

Research output: Contribution to journalArticle

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