Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation

Francesca Cella Zanacchi, Zeno Lavagnino, Mario Faretta, Laura Furia, Alberto Diaspro

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Light-sheet microscopy is a useful tool for performing biological investigations of thick samples and it has recently been demonstrated that it can also act as a suitable architecture for super-resolution imaging of thick biological samples by means of individual molecule localization. However, imaging in depth is still limited since it suffers from a reduction in image quality caused by scattering effects. This paper sets out to investigate the advantages of non-linear photoactivation implemented in a selective plane illumination configuration when imaging scattering samples. In particular, two-photon excitation is proven to improve imaging capabilities in terms of imaging depth and is expected to reduce light-sample interactions and sample photo-damage. Here, two-photon photoactivation is coupled to individual molecule localization methods based on light-sheet illumination (IML-SPIM), allowing super-resolution imaging of nuclear pH2AX in NB4 cells.

Original languageEnglish
Article numbere67667
JournalPLoS One
Volume8
Issue number7
DOIs
Publication statusPublished - Jul 2 2013

Fingerprint

Photons
image analysis
Lighting
Imaging techniques
Light
lighting
Microscopy
sampling
Scattering
Molecules
Image quality
photons
microscopy
Microscopic examination
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation. / Cella Zanacchi, Francesca; Lavagnino, Zeno; Faretta, Mario; Furia, Laura; Diaspro, Alberto.

In: PLoS One, Vol. 8, No. 7, e67667, 02.07.2013.

Research output: Contribution to journalArticle

Cella Zanacchi, F, Lavagnino, Z, Faretta, M, Furia, L & Diaspro, A 2013, 'Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation', PLoS One, vol. 8, no. 7, e67667. https://doi.org/10.1371/journal.pone.0067667
Cella Zanacchi, Francesca ; Lavagnino, Zeno ; Faretta, Mario ; Furia, Laura ; Diaspro, Alberto. / Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation. In: PLoS One. 2013 ; Vol. 8, No. 7.
@article{451300b255bb4604a8e91b5ac91e2c96,
title = "Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation",
abstract = "Light-sheet microscopy is a useful tool for performing biological investigations of thick samples and it has recently been demonstrated that it can also act as a suitable architecture for super-resolution imaging of thick biological samples by means of individual molecule localization. However, imaging in depth is still limited since it suffers from a reduction in image quality caused by scattering effects. This paper sets out to investigate the advantages of non-linear photoactivation implemented in a selective plane illumination configuration when imaging scattering samples. In particular, two-photon excitation is proven to improve imaging capabilities in terms of imaging depth and is expected to reduce light-sample interactions and sample photo-damage. Here, two-photon photoactivation is coupled to individual molecule localization methods based on light-sheet illumination (IML-SPIM), allowing super-resolution imaging of nuclear pH2AX in NB4 cells.",
author = "{Cella Zanacchi}, Francesca and Zeno Lavagnino and Mario Faretta and Laura Furia and Alberto Diaspro",
year = "2013",
month = "7",
day = "2",
doi = "10.1371/journal.pone.0067667",
language = "English",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

TY - JOUR

T1 - Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation

AU - Cella Zanacchi, Francesca

AU - Lavagnino, Zeno

AU - Faretta, Mario

AU - Furia, Laura

AU - Diaspro, Alberto

PY - 2013/7/2

Y1 - 2013/7/2

N2 - Light-sheet microscopy is a useful tool for performing biological investigations of thick samples and it has recently been demonstrated that it can also act as a suitable architecture for super-resolution imaging of thick biological samples by means of individual molecule localization. However, imaging in depth is still limited since it suffers from a reduction in image quality caused by scattering effects. This paper sets out to investigate the advantages of non-linear photoactivation implemented in a selective plane illumination configuration when imaging scattering samples. In particular, two-photon excitation is proven to improve imaging capabilities in terms of imaging depth and is expected to reduce light-sample interactions and sample photo-damage. Here, two-photon photoactivation is coupled to individual molecule localization methods based on light-sheet illumination (IML-SPIM), allowing super-resolution imaging of nuclear pH2AX in NB4 cells.

AB - Light-sheet microscopy is a useful tool for performing biological investigations of thick samples and it has recently been demonstrated that it can also act as a suitable architecture for super-resolution imaging of thick biological samples by means of individual molecule localization. However, imaging in depth is still limited since it suffers from a reduction in image quality caused by scattering effects. This paper sets out to investigate the advantages of non-linear photoactivation implemented in a selective plane illumination configuration when imaging scattering samples. In particular, two-photon excitation is proven to improve imaging capabilities in terms of imaging depth and is expected to reduce light-sample interactions and sample photo-damage. Here, two-photon photoactivation is coupled to individual molecule localization methods based on light-sheet illumination (IML-SPIM), allowing super-resolution imaging of nuclear pH2AX in NB4 cells.

UR - http://www.scopus.com/inward/record.url?scp=84879747564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879747564&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0067667

DO - 10.1371/journal.pone.0067667

M3 - Article

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 7

M1 - e67667

ER -