Abstract
Aims: Circumstantial evidence suggests that genetic changes may lead to tumor progression within the myxoid liposarcoma tumors (MLTs) carrying non- random chromosomal translocation t(12;16). Methods: To address this subject an immunophenotypic analysis, applying antibodies against proteins encoded by TP53, MDM2 and CDK4 genes, complemented by molecular analysis of eight suitable cases, was performed on 104 consecutive cases. Chromosomal translocations were assessed either by cytogenetic analysis or by RT-PCR in 9 suitable cases and chimeric transcripts were found in all cases but two pleomorphic liposarcomas. Results: Based on immunophenotyping and tumor site, the case material consisted of three groups. The first one was made up of 92 non-retroperitoneal cases carrying a null p53, mdm2, cdk4 immunophenotype, which remained unchanged over the time of recurrences and along the gamut of histologic subtypes. The second group was represented by five p53+, mdm2-, cdk4- non-retroperitoneal cases, 4 of which were further analysed by PCR- SSCP for p53 mutation. The immunophenotipic profile of these cases, complemented by the molecular findings, supported a role of TP53 in tumor progression in three high-grade MLTs. The third group, consisting of 7 retroperitoneal cases, showed a heterogeneous immunophenotype, sharing immunophenotypic and molecular features with the well-differentiated/evoluted (dedifferentiated) liposarcoma group. Conclusions: TP53 mutations seem to play a role in tumor progression in a few cases of MLTs (2.8%) showing more aggressive histologic characteristics. The unexpected finding that a number of retroperitoneal LMTs display the immunophenotypic profile of the well differentiated/evoluted (dedifferentiated) liposarcomas, deserves further investigation.
| Original language | English |
|---|---|
| Pages (from-to) | 571-577 |
| Number of pages | 7 |
| Journal | Tumori |
| Volume | 84 |
| Issue number | 5 |
| Publication status | Published - Sep 1998 |
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Keywords
- cdk4
- Cytogenetics
- DNA and RNA analysis
- Immunophenotyping
- mdm2
- Myxoid liposarcoma tumors
- p53
ASJC Scopus subject areas
- Cancer Research
Cite this
Limited role of TP53 and TP53-related genes in myxoid liposarcoma. / Pilotti, Silvana; Lavarino, Cinzia; Mezzelani, Alessandra; Della Torre, Gabriella; Minoletti, Fabiola; Sozzi, Gabriella; Azzarelli, Alberto; Rilke, Franco; Pierotti, Marco A.
In: Tumori, Vol. 84, No. 5, 09.1998, p. 571-577.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Limited role of TP53 and TP53-related genes in myxoid liposarcoma
AU - Pilotti, Silvana
AU - Lavarino, Cinzia
AU - Mezzelani, Alessandra
AU - Della Torre, Gabriella
AU - Minoletti, Fabiola
AU - Sozzi, Gabriella
AU - Azzarelli, Alberto
AU - Rilke, Franco
AU - Pierotti, Marco A.
PY - 1998/9
Y1 - 1998/9
N2 - Aims: Circumstantial evidence suggests that genetic changes may lead to tumor progression within the myxoid liposarcoma tumors (MLTs) carrying non- random chromosomal translocation t(12;16). Methods: To address this subject an immunophenotypic analysis, applying antibodies against proteins encoded by TP53, MDM2 and CDK4 genes, complemented by molecular analysis of eight suitable cases, was performed on 104 consecutive cases. Chromosomal translocations were assessed either by cytogenetic analysis or by RT-PCR in 9 suitable cases and chimeric transcripts were found in all cases but two pleomorphic liposarcomas. Results: Based on immunophenotyping and tumor site, the case material consisted of three groups. The first one was made up of 92 non-retroperitoneal cases carrying a null p53, mdm2, cdk4 immunophenotype, which remained unchanged over the time of recurrences and along the gamut of histologic subtypes. The second group was represented by five p53+, mdm2-, cdk4- non-retroperitoneal cases, 4 of which were further analysed by PCR- SSCP for p53 mutation. The immunophenotipic profile of these cases, complemented by the molecular findings, supported a role of TP53 in tumor progression in three high-grade MLTs. The third group, consisting of 7 retroperitoneal cases, showed a heterogeneous immunophenotype, sharing immunophenotypic and molecular features with the well-differentiated/evoluted (dedifferentiated) liposarcoma group. Conclusions: TP53 mutations seem to play a role in tumor progression in a few cases of MLTs (2.8%) showing more aggressive histologic characteristics. The unexpected finding that a number of retroperitoneal LMTs display the immunophenotypic profile of the well differentiated/evoluted (dedifferentiated) liposarcomas, deserves further investigation.
AB - Aims: Circumstantial evidence suggests that genetic changes may lead to tumor progression within the myxoid liposarcoma tumors (MLTs) carrying non- random chromosomal translocation t(12;16). Methods: To address this subject an immunophenotypic analysis, applying antibodies against proteins encoded by TP53, MDM2 and CDK4 genes, complemented by molecular analysis of eight suitable cases, was performed on 104 consecutive cases. Chromosomal translocations were assessed either by cytogenetic analysis or by RT-PCR in 9 suitable cases and chimeric transcripts were found in all cases but two pleomorphic liposarcomas. Results: Based on immunophenotyping and tumor site, the case material consisted of three groups. The first one was made up of 92 non-retroperitoneal cases carrying a null p53, mdm2, cdk4 immunophenotype, which remained unchanged over the time of recurrences and along the gamut of histologic subtypes. The second group was represented by five p53+, mdm2-, cdk4- non-retroperitoneal cases, 4 of which were further analysed by PCR- SSCP for p53 mutation. The immunophenotipic profile of these cases, complemented by the molecular findings, supported a role of TP53 in tumor progression in three high-grade MLTs. The third group, consisting of 7 retroperitoneal cases, showed a heterogeneous immunophenotype, sharing immunophenotypic and molecular features with the well-differentiated/evoluted (dedifferentiated) liposarcoma group. Conclusions: TP53 mutations seem to play a role in tumor progression in a few cases of MLTs (2.8%) showing more aggressive histologic characteristics. The unexpected finding that a number of retroperitoneal LMTs display the immunophenotypic profile of the well differentiated/evoluted (dedifferentiated) liposarcomas, deserves further investigation.
KW - cdk4
KW - Cytogenetics
KW - DNA and RNA analysis
KW - Immunophenotyping
KW - mdm2
KW - Myxoid liposarcoma tumors
KW - p53
UR - http://www.scopus.com/inward/record.url?scp=0031672399&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031672399&partnerID=8YFLogxK
M3 - Article
C2 - 9862519
AN - SCOPUS:0031672399
VL - 84
SP - 571
EP - 577
JO - Tumori
JF - Tumori
SN - 0300-8916
IS - 5
ER -