Limited role of TP53 and TP53-related genes in myxoid liposarcoma

Silvana Pilotti, Cinzia Lavarino, Alessandra Mezzelani, Gabriella Della Torre, Fabiola Minoletti, Gabriella Sozzi, Alberto Azzarelli, Franco Rilke, Marco A. Pierotti

Research output: Contribution to journalArticle

Abstract

Aims: Circumstantial evidence suggests that genetic changes may lead to tumor progression within the myxoid liposarcoma tumors (MLTs) carrying non- random chromosomal translocation t(12;16). Methods: To address this subject an immunophenotypic analysis, applying antibodies against proteins encoded by TP53, MDM2 and CDK4 genes, complemented by molecular analysis of eight suitable cases, was performed on 104 consecutive cases. Chromosomal translocations were assessed either by cytogenetic analysis or by RT-PCR in 9 suitable cases and chimeric transcripts were found in all cases but two pleomorphic liposarcomas. Results: Based on immunophenotyping and tumor site, the case material consisted of three groups. The first one was made up of 92 non-retroperitoneal cases carrying a null p53, mdm2, cdk4 immunophenotype, which remained unchanged over the time of recurrences and along the gamut of histologic subtypes. The second group was represented by five p53+, mdm2-, cdk4- non-retroperitoneal cases, 4 of which were further analysed by PCR- SSCP for p53 mutation. The immunophenotipic profile of these cases, complemented by the molecular findings, supported a role of TP53 in tumor progression in three high-grade MLTs. The third group, consisting of 7 retroperitoneal cases, showed a heterogeneous immunophenotype, sharing immunophenotypic and molecular features with the well-differentiated/evoluted (dedifferentiated) liposarcoma group. Conclusions: TP53 mutations seem to play a role in tumor progression in a few cases of MLTs (2.8%) showing more aggressive histologic characteristics. The unexpected finding that a number of retroperitoneal LMTs display the immunophenotypic profile of the well differentiated/evoluted (dedifferentiated) liposarcomas, deserves further investigation.

Original languageEnglish
Pages (from-to)571-577
Number of pages7
JournalTumori
Volume84
Issue number5
Publication statusPublished - Sep 1998

Fingerprint

Myxoid Liposarcoma
p53 Genes
Liposarcoma
Neoplasms
Genetic Translocation
Tumor Suppressor Protein p53
Single-Stranded Conformational Polymorphism
Immunophenotyping
Polymerase Chain Reaction
Mutation
Cytogenetic Analysis
Recurrence
Antibodies

Keywords

  • cdk4
  • Cytogenetics
  • DNA and RNA analysis
  • Immunophenotyping
  • mdm2
  • Myxoid liposarcoma tumors
  • p53

ASJC Scopus subject areas

  • Cancer Research

Cite this

Pilotti, S., Lavarino, C., Mezzelani, A., Della Torre, G., Minoletti, F., Sozzi, G., ... Pierotti, M. A. (1998). Limited role of TP53 and TP53-related genes in myxoid liposarcoma. Tumori, 84(5), 571-577.

Limited role of TP53 and TP53-related genes in myxoid liposarcoma. / Pilotti, Silvana; Lavarino, Cinzia; Mezzelani, Alessandra; Della Torre, Gabriella; Minoletti, Fabiola; Sozzi, Gabriella; Azzarelli, Alberto; Rilke, Franco; Pierotti, Marco A.

In: Tumori, Vol. 84, No. 5, 09.1998, p. 571-577.

Research output: Contribution to journalArticle

Pilotti, S, Lavarino, C, Mezzelani, A, Della Torre, G, Minoletti, F, Sozzi, G, Azzarelli, A, Rilke, F & Pierotti, MA 1998, 'Limited role of TP53 and TP53-related genes in myxoid liposarcoma', Tumori, vol. 84, no. 5, pp. 571-577.
Pilotti S, Lavarino C, Mezzelani A, Della Torre G, Minoletti F, Sozzi G et al. Limited role of TP53 and TP53-related genes in myxoid liposarcoma. Tumori. 1998 Sep;84(5):571-577.
Pilotti, Silvana ; Lavarino, Cinzia ; Mezzelani, Alessandra ; Della Torre, Gabriella ; Minoletti, Fabiola ; Sozzi, Gabriella ; Azzarelli, Alberto ; Rilke, Franco ; Pierotti, Marco A. / Limited role of TP53 and TP53-related genes in myxoid liposarcoma. In: Tumori. 1998 ; Vol. 84, No. 5. pp. 571-577.
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abstract = "Aims: Circumstantial evidence suggests that genetic changes may lead to tumor progression within the myxoid liposarcoma tumors (MLTs) carrying non- random chromosomal translocation t(12;16). Methods: To address this subject an immunophenotypic analysis, applying antibodies against proteins encoded by TP53, MDM2 and CDK4 genes, complemented by molecular analysis of eight suitable cases, was performed on 104 consecutive cases. Chromosomal translocations were assessed either by cytogenetic analysis or by RT-PCR in 9 suitable cases and chimeric transcripts were found in all cases but two pleomorphic liposarcomas. Results: Based on immunophenotyping and tumor site, the case material consisted of three groups. The first one was made up of 92 non-retroperitoneal cases carrying a null p53, mdm2, cdk4 immunophenotype, which remained unchanged over the time of recurrences and along the gamut of histologic subtypes. The second group was represented by five p53+, mdm2-, cdk4- non-retroperitoneal cases, 4 of which were further analysed by PCR- SSCP for p53 mutation. The immunophenotipic profile of these cases, complemented by the molecular findings, supported a role of TP53 in tumor progression in three high-grade MLTs. The third group, consisting of 7 retroperitoneal cases, showed a heterogeneous immunophenotype, sharing immunophenotypic and molecular features with the well-differentiated/evoluted (dedifferentiated) liposarcoma group. Conclusions: TP53 mutations seem to play a role in tumor progression in a few cases of MLTs (2.8{\%}) showing more aggressive histologic characteristics. The unexpected finding that a number of retroperitoneal LMTs display the immunophenotypic profile of the well differentiated/evoluted (dedifferentiated) liposarcomas, deserves further investigation.",
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AU - Minoletti, Fabiola

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AB - Aims: Circumstantial evidence suggests that genetic changes may lead to tumor progression within the myxoid liposarcoma tumors (MLTs) carrying non- random chromosomal translocation t(12;16). Methods: To address this subject an immunophenotypic analysis, applying antibodies against proteins encoded by TP53, MDM2 and CDK4 genes, complemented by molecular analysis of eight suitable cases, was performed on 104 consecutive cases. Chromosomal translocations were assessed either by cytogenetic analysis or by RT-PCR in 9 suitable cases and chimeric transcripts were found in all cases but two pleomorphic liposarcomas. Results: Based on immunophenotyping and tumor site, the case material consisted of three groups. The first one was made up of 92 non-retroperitoneal cases carrying a null p53, mdm2, cdk4 immunophenotype, which remained unchanged over the time of recurrences and along the gamut of histologic subtypes. The second group was represented by five p53+, mdm2-, cdk4- non-retroperitoneal cases, 4 of which were further analysed by PCR- SSCP for p53 mutation. The immunophenotipic profile of these cases, complemented by the molecular findings, supported a role of TP53 in tumor progression in three high-grade MLTs. The third group, consisting of 7 retroperitoneal cases, showed a heterogeneous immunophenotype, sharing immunophenotypic and molecular features with the well-differentiated/evoluted (dedifferentiated) liposarcoma group. Conclusions: TP53 mutations seem to play a role in tumor progression in a few cases of MLTs (2.8%) showing more aggressive histologic characteristics. The unexpected finding that a number of retroperitoneal LMTs display the immunophenotypic profile of the well differentiated/evoluted (dedifferentiated) liposarcomas, deserves further investigation.

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