Linkage disequilibrium analysis of the human adenosine deaminase (ADA) gene provides evidence for a lack of correlation between hot spots of equal and unequal homologous recombination

Fulvio Cruciani, Laura Bernardini, Piero Santolamazza, David Modiano, Antonio Torroni, Rosaria Scozzari

Research output: Contribution to journalArticle

Abstract

The linkage disequilibrium (LD) pattern within the adenosine deaminase (ADA) gene was analyzed by studying 13 polymorphic loci in 137 families from two European and three African populations. Evidence for the presence of a 12-kb meiotic crossover hot spot, spanning part of the first and the second intron and flanked by regions of reduced recombination activity, was obtained. Moreover, segregation analysis of 113 informative meioses revealed two recombination events that are internal or overlap the 12-kb region, thus suggesting a recombination rate for the hot-spot region about 50-fold higher than the mean rate across the human genome. Within the hot spot, a 144-bp palindromic sequence was also identified and its possible involvement in the recombination process is discussed. The 12-kb region characterized by the low degree of LD does not include the 3.2-kb region that is deleted, as a result of recurrent unequal homologous recombination between two Alu elements, in patients affected by autosomal severe combined immunodeficiency. This observation provides the first evidence for an absence of correlation between hot spots of equal and unequal homologous recombination.

Original languageEnglish
Pages (from-to)20-33
Number of pages14
JournalGenomics
Volume82
Issue number1
DOIs
Publication statusPublished - Jul 1 2003

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Keywords

  • ADA gene
  • Adenosine deaminase
  • Linkage disequilibrium
  • Recombination hot spot
  • Severe combined immunodeficiency

ASJC Scopus subject areas

  • Genetics

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