TY - JOUR
T1 - Linkage disequilibrium and haplotype mapping of a skin cancer susceptibility locus in outbred mice
AU - Peissel, Bernard
AU - Zaffaroni, Daniela
AU - Zanesi, Nicola
AU - Zedda, Ignazio
AU - Manenti, Giacomo
AU - Rebessi, Simonetta
AU - Pazzaglia, Simonetta
AU - Doria, Gino
AU - Covelli, Vincenzo
AU - Dragani, Tommaso A.
AU - Saran, Anna
PY - 2000
Y1 - 2000
N2 - Car-R (carcinogenesis-resistant) and Car-S (carcinogenesis-susceptible) outbred mice, obtained by phenotypic selection from an initial intercross of eight inbred strains, show a >100fold difference in their susceptibility to two-stage skin tumorigenesis. We found that the lines carry a high degree of genetic polymorphism, with an average heterozygosity of 0.39. This polymorphism allowed the use of linkage disequilibrium (LD) and haplotype analysis for the mapping of a skin cancer modifier locus on Chr 7, in a short region of 6 cM, around the Tyr gene. Car-S mice inherited the susceptibility allele at this locus from the A/J, BALB/c, SJL/J, and SWR/J strains. Our results demonstrate the feasibility and usefulness of mapping disease genes by LD in phenotypically selected, genetically heterogeneous animals.
AB - Car-R (carcinogenesis-resistant) and Car-S (carcinogenesis-susceptible) outbred mice, obtained by phenotypic selection from an initial intercross of eight inbred strains, show a >100fold difference in their susceptibility to two-stage skin tumorigenesis. We found that the lines carry a high degree of genetic polymorphism, with an average heterozygosity of 0.39. This polymorphism allowed the use of linkage disequilibrium (LD) and haplotype analysis for the mapping of a skin cancer modifier locus on Chr 7, in a short region of 6 cM, around the Tyr gene. Car-S mice inherited the susceptibility allele at this locus from the A/J, BALB/c, SJL/J, and SWR/J strains. Our results demonstrate the feasibility and usefulness of mapping disease genes by LD in phenotypically selected, genetically heterogeneous animals.
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M3 - Article
C2 - 11063253
AN - SCOPUS:0033777840
VL - 11
SP - 979
EP - 981
JO - Mammalian Genome
JF - Mammalian Genome
SN - 0938-8990
IS - 11
ER -