Linkage disequilibrium and physical mapping of Pas1 in mice

Giacomo Manenti, Amanda Stafford, Laura De Gregorio, Manuela Gariboldi, F. Stefania Falvella, Philip Avner, Tommaso A. Dragani

Research output: Contribution to journalArticle

Abstract

By using linkage disequilibrium (LD) analysis in 21 strains of known susceptibility to lung cancer and by assembling a YAC contig, we mapped to a ~ 1.5 Mb region on distal mouse chromosome 6 the Pas1 locus, the major determinant of lung cancer predisposition in mice. Our results, on the basis of haplotype and phenetic analysis, suggest that the Pasl(s) susceptibility allele is shared by several mouse-inbred strains of independent origin, which show either high or intermediate predisposition to lung tumorigenesis. Therefore, the Pas1(s) allele is probably derived from an ancestral mouse rather than from independent mutations of the same gene. We showed the feasibility of LD in common inbred strains for the fine mapping of disease loci, and provided the biological basis and the reagents for the cloning of the Pas1 gene.

Original languageEnglish
Pages (from-to)639-646
Number of pages8
JournalGenome Research
Volume9
Issue number7
Publication statusPublished - Jul 1999

ASJC Scopus subject areas

  • Genetics

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    Manenti, G., Stafford, A., De Gregorio, L., Gariboldi, M., Falvella, F. S., Avner, P., & Dragani, T. A. (1999). Linkage disequilibrium and physical mapping of Pas1 in mice. Genome Research, 9(7), 639-646.