Abstract
We have discovered a new α-melanocyte stimulating hormone (α-MSH)/peroxisome proliferator activated receptor-γ (PPAR-γ) connection in B16-F10 cells. Both PPAR-γ up-regulation and its induction as an active transcription factor were observed in response to α-MSH. The α-MSH/PPAR-γ connection influenced both pigmentation and proliferation. The forskolin-stimulated cAMP/PKA pathway was not able to induce either PPAR-γ translocation into the nucleus or PPAR-γ transcriptional activity. As the melanocortin-1 receptor, the specific receptor for the α-MSH, is a G-protein coupled receptor, we wondered whether the phosphatidylinositol [PI(4,5)P2/PLCβ] signal pathway was involved in mediating the α-MSH-dependent PPAR-γ activation. Employing inhibitors of PI(4,5)P2/PLCβ pathway, the results of our experiments suggested that this pathway was promoted by α-MSH and that α-MSH played a role in mediating PPAR-γ activation. We have demonstrated, for the first time, that α-MSH induces the PI(4,5)P2/PLCβ pathway, through analysis of the basic steps of the pathway. The α-MSH effect on PPAR-γ was independent of animal species and was not correlated with the physio-pathological status.
Original language | English |
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Pages (from-to) | 113-127 |
Number of pages | 15 |
Journal | Pigment Cell and Melanoma Research |
Volume | 26 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2013 |
Keywords
- α-melanocyte stimulating hormone
- B16-F10
- Calcium fluxes
- Peroxisome proliferator activated receptor-γ
- PI(4,5)P
- PLC pathway
ASJC Scopus subject areas
- Dermatology
- Oncology
- Biochemistry, Genetics and Molecular Biology(all)