LIPA gene mutations affect the composition of lipoproteins: Enrichment in ACAT-derived cholesteryl esters

L. Arnaboldi, A. Ossoli, E. Giorgio, L. Pisciotta, T. Lucchi, L. Grigore, C. Pavanello, A. Granata, A. Pasta, B. Arosio, D. Azzolino, A. Baragetti, S. Castelnuovo, A. Corsini, A.L. Catapano, L. Calabresi, M. Gomaraschi

Research output: Contribution to journalArticlepeer-review

Abstract

Background and aims: Cholesteryl ester storage disease (CESD) due to LIPA gene mutations is characterized by hepatic steatosis, hypercholesterolemia and hypoalphalipoproteinemia, exposing affected patients to an increased cardiovascular risk. Further insights into the impact of LIPA gene mutations on lipid/lipoprotein metabolism are limited. Aim of the study was to investigate the effect of carrying one or two mutant LIPA alleles on lipoprotein composition and function. Methods: Lipoproteins were isolated from 6 adult CESD patients, 5 relatives carrying one mutant LIPA allele (carriers) and 12 sex/age matched controls. Lipid profile, lipoprotein mass composition and the fatty acid distribution of cholesteryl esters (CEs) were assessed. HDL function was evaluated as the ability to promote nitric oxide release by endothelial cells. Results: Despite the lipid-lowering therapy, total cholesterol, LDL-cholesterol and triglycerides were increased in CESD patients compared to controls, while HDL-cholesterol was reduced. Carriers also displayed elevated total and LDL-cholesterol. Very low and intermediate density lipoproteins from CESD patients and carriers were enriched in CEs compared to the control ones, with a concomitant reduction of triglycerides. Fatty acid composition of CEs in serum and lipoproteins showed a depletion of linoleate content in CESD patients, due to the reduced LCAT activity. In CESD HDL, fatty acid distribution of CEs was shifted towards saturated ones, if compared to control HDL. The changes in HDL composition did not affect HDL ability to promote nitric oxide release by endothelial cells. Conclusions: LIPA gene mutations significantly affected plasma levels and lipid composition of lipoproteins, likely contributing to the increased cardiovascular risk of affected patients. © 2020 Elsevier B.V.
Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalAtherosclerosis
Volume297
DOIs
Publication statusPublished - 2020

Keywords

  • Cholesteryl ester storage disease
  • Lipoproteins
  • Lysosomal acid lipase
  • alanine aminotransferase
  • apolipoprotein A1
  • apolipoprotein B
  • aspartate aminotransferase
  • cholesterol
  • cholesterol acyltransferase
  • cholesterol ester
  • gamma glutamyltransferase
  • high density lipoprotein
  • high density lipoprotein cholesterol
  • intermediate density lipoprotein
  • linoleic acid
  • lipoprotein
  • low density lipoprotein cholesterol
  • nitric oxide
  • phosphatidylcholine sterol acyltransferase
  • triacylglycerol
  • very low density lipoprotein
  • adult
  • alanine aminotransferase blood level
  • allele
  • Article
  • aspartate aminotransferase blood level
  • blood sampling
  • cholesterol blood level
  • cholesterol ester storage disease
  • clinical article
  • controlled study
  • female
  • gamma glutamyl transferase blood level
  • human
  • lipa gene
  • lipid composition
  • lipid fingerprinting
  • male
  • mutator gene
  • priority journal
  • triacylglycerol blood level

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