Lipid characteristics of RSV-transformed Balb/c 3T3 cell lines with different spontaneous metastatic potentials

Lido Calorini, Anna Fallani, Donatella Tombaccini, Emanuela Barletta, Gabriele Mugnai, Maria F. Di Renzo, Paolo M. Comoglio, Salvatore Ruggieri

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To determine whether a metastatic phenotype may be corelated with a characteristic lipid pattern, we compared the lipid composition of low metastasizing Balb/c 3T3 cells transformed by the B77 strain of Rous sarcoma virus (B77-3T3 cells) with that of a subclone isolated by growth in 0.6% agar, the B77-AA6 cells, which exhibit a high capacity for spontaneous metastasis. B77-3T3 cells revealed characteristics in their lipid composition common to other systems of transformed cells, i.e., an accumulation of ether-linked lipids, a reduction of the more complex gangliosides, an increase of oleic acid (18:1) and a decrease of arachidonic (20:4) and C22 polyunsaturated fatty acids in phospholipids. High metastatic B77-AA6 cells showed: a) an even more marked decrease of complex gangliosides; b) a more pronounced increase of 18:1 and decrease of 20:4 and 22 polyunsaturated fatty acids in certain phospholipid classes; and c) a higher percentage of alkyl-acyl subfractions in both phosphatidylcholine and phosphatidylethanolamine than B77-3T3 cells. Comparing the data for other systems of metastatic cells with those of lipid studies of spontaneously metastasizing B77-AA6 cell system leads us to conclude that the metastatic phenotype is characterized by a change in ether-linked lipids, rather than in fatty acids.

Original languageEnglish
Pages (from-to)685-690
Number of pages6
JournalLipids
Volume24
Issue number8
DOIs
Publication statusPublished - Aug 1989

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3T3 Cells
Cells
cell lines
Lipids
Cell Line
lipids
Gangliosides
cells
Unsaturated Fatty Acids
Ether
gangliosides
Phospholipids
lipid composition
Phenotype
Rous sarcoma virus
polyunsaturated fatty acids
ethers
phospholipids
Oleic Acid
Chemical analysis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science

Cite this

Calorini, L., Fallani, A., Tombaccini, D., Barletta, E., Mugnai, G., Di Renzo, M. F., ... Ruggieri, S. (1989). Lipid characteristics of RSV-transformed Balb/c 3T3 cell lines with different spontaneous metastatic potentials. Lipids, 24(8), 685-690. https://doi.org/10.1007/BF02535205

Lipid characteristics of RSV-transformed Balb/c 3T3 cell lines with different spontaneous metastatic potentials. / Calorini, Lido; Fallani, Anna; Tombaccini, Donatella; Barletta, Emanuela; Mugnai, Gabriele; Di Renzo, Maria F.; Comoglio, Paolo M.; Ruggieri, Salvatore.

In: Lipids, Vol. 24, No. 8, 08.1989, p. 685-690.

Research output: Contribution to journalArticle

Calorini, L, Fallani, A, Tombaccini, D, Barletta, E, Mugnai, G, Di Renzo, MF, Comoglio, PM & Ruggieri, S 1989, 'Lipid characteristics of RSV-transformed Balb/c 3T3 cell lines with different spontaneous metastatic potentials', Lipids, vol. 24, no. 8, pp. 685-690. https://doi.org/10.1007/BF02535205
Calorini, Lido ; Fallani, Anna ; Tombaccini, Donatella ; Barletta, Emanuela ; Mugnai, Gabriele ; Di Renzo, Maria F. ; Comoglio, Paolo M. ; Ruggieri, Salvatore. / Lipid characteristics of RSV-transformed Balb/c 3T3 cell lines with different spontaneous metastatic potentials. In: Lipids. 1989 ; Vol. 24, No. 8. pp. 685-690.
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