Lipid interaction of Tetanus neurotoxin A calorimetric and fluorescence spectroscopy study

Emanuela Calappi, Massimo Masserini, Giampietro Schiavo, Cesare Montecucco, Guido Tettamanti

Research output: Contribution to journalArticlepeer-review

Abstract

The interaction of Tetunus toxin with phospholipid vesicles containing gangliosides (GD1a, GD1b or GT1b) or phosphatidic acid has been investigated at neutral or acidic pH. Change in the thermotropic properties of the vesicles occurred only after addition of the toxin at acidic pH, and led to surface binding or membrane insertion of the protein, dependent on the physical state of the membrane. Most remarkably, toxin addition at acidic pH to dipalmitoyl-phosphatidylcholine vesicles containing GT1b ganglioside, caused formation of ganglioside microdomains on the vesicle surface.

Original languageEnglish
Pages (from-to)107-110
Number of pages4
JournalFEBS Letters
Volume309
Issue number2
DOIs
Publication statusPublished - Sep 7 1992

Keywords

  • Ganglioside microdomain
  • Tetanus toxin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

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