Lipidomic investigations for the characterization of circulating serum lipids in multiple sclerosis

Piero Del Boccio, Damiana Pieragostino, Maria Di Ioia, Francesca Petrucci, Alessandra Lugaresi, Giovanna De Luca, Domenico Gambi, Marco Onofrj, Carmine Di Ilio, Paolo Sacchetta, Andrea Urbani

Research output: Contribution to journalArticlepeer-review

Abstract

Multiple Sclerosis (MS) is a neurodegenerative autoimmune demyelinating disease affecting young adults. The aetiology still remains a mystery and diagnosis is impaired by the lack of defined molecular markers. Autoimmune response remains the main topic under investigation and recent studies suggest additional non-proteic mediators of brain inflammation such as lipids. We carried out an LC-MS based lipidomics approach to highlight serum lipids profiling in MS. Method was optimised and applied in a preliminary clinical cross-sectional investigation of MS patients vs Healthy Controls (HC) and patients with Other Neurological Diseases (OND). Ten significant metabolites were highlighted and tentatively identified by accurate mass and MS/MS experiments. Our most relevant data show altered level of lyso-glycerophosphatidylcholine (lysoPC) and glycerophosphatidylcholine (PC) species. Total lysoPC/PC ratio showed significant decrease in pathological groups (MS, OND) and, in addition, MS subjects had a relevant decrease of this ratio also in respect to OND. These findings suggest that there may be an altered phospholipid metabolism in MS that can be evaluated in serum. Some of these features are distinctive and may be considered specific for MS. Our lipidomics data show, for the first time, evidence in serum of a relationship between LysoPC/PC ratio and MS.

Original languageEnglish
Pages (from-to)2826-2836
Number of pages11
JournalJournal of Proteomics
Volume74
Issue number12
DOIs
Publication statusPublished - Nov 18 2011

Keywords

  • Biomarker
  • Lipidomics
  • Mass spectrometry
  • Multiple sclerosis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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