Lipids in Aspergillus flavus-maize interaction

Marzia Scarpari, Marta Punelli, Valeria Scala, Marco Zaccaria, Chiara Nobili, Matteo Ludovici, Emanuela Camera, Anna A. Fabbri, Massimo Reverberi, Corrado Fanelli

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In some filamentous fungi, the pathways related to the oxidative stress and oxylipins production are involved both in the process of host-recognition and in the pathogenic phase. In fact, recent studies have shown that the production of oxylipins in filamentous fungi, yeasts and chromists is also related to the development of the organism itself and to mechanisms of communication with the host at the cellular level. The oxylipins, also produced by the host during defense reactions, are able to induce sporulation and to regulate the biosynthesis of mycotoxins in several pathogenic fungi. In A. flavus, the oxylipins play a crucial role as signals for regulating the biosynthesis of aflatoxins, the conidiogenesis and the formation of sclerotia. To investigate the involvement of an oxylipins based cross-talk into Z. mays and A. flavus interaction, we analyzed the oxylipins profile of the wild type strain and of three mutants of A. flavus that are deleted at the Aflox1 gene level also during maize kernel invasion. A lipidomic approach has been addressed through the use of LC-ToF-MS, followed by a statistical analysis of the principal components (PCA). The results showed the existence of a difference between the oxylipins profile generated by the WT and the mutants onto challenged maize. In relation to this, aflatoxin synthesis which is largely hampered in vitro, is intriguingly restored. These results highlight the important role of maize oxylipin in driving secondary metabolism in A. flavus.

Original languageEnglish
JournalFrontiers in Microbiology
Volume5
Issue numberFEB
DOIs
Publication statusPublished - 2014

Fingerprint

Oxylipins
Aspergillus flavus
Zea mays
Lipids
Fungi
Aflatoxins
Secondary Metabolism
Mycotoxins
Principal Component Analysis
Oxidative Stress
Yeasts

Keywords

  • Aflatoxins
  • Lipidomic
  • Lipoxygenase
  • Maize kernels
  • Reverse genetic

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Scarpari, M., Punelli, M., Scala, V., Zaccaria, M., Nobili, C., Ludovici, M., ... Fanelli, C. (2014). Lipids in Aspergillus flavus-maize interaction. Frontiers in Microbiology, 5(FEB). https://doi.org/10.3389/fmicb.2014.00074

Lipids in Aspergillus flavus-maize interaction. / Scarpari, Marzia; Punelli, Marta; Scala, Valeria; Zaccaria, Marco; Nobili, Chiara; Ludovici, Matteo; Camera, Emanuela; Fabbri, Anna A.; Reverberi, Massimo; Fanelli, Corrado.

In: Frontiers in Microbiology, Vol. 5, No. FEB, 2014.

Research output: Contribution to journalArticle

Scarpari, M, Punelli, M, Scala, V, Zaccaria, M, Nobili, C, Ludovici, M, Camera, E, Fabbri, AA, Reverberi, M & Fanelli, C 2014, 'Lipids in Aspergillus flavus-maize interaction', Frontiers in Microbiology, vol. 5, no. FEB. https://doi.org/10.3389/fmicb.2014.00074
Scarpari M, Punelli M, Scala V, Zaccaria M, Nobili C, Ludovici M et al. Lipids in Aspergillus flavus-maize interaction. Frontiers in Microbiology. 2014;5(FEB). https://doi.org/10.3389/fmicb.2014.00074
Scarpari, Marzia ; Punelli, Marta ; Scala, Valeria ; Zaccaria, Marco ; Nobili, Chiara ; Ludovici, Matteo ; Camera, Emanuela ; Fabbri, Anna A. ; Reverberi, Massimo ; Fanelli, Corrado. / Lipids in Aspergillus flavus-maize interaction. In: Frontiers in Microbiology. 2014 ; Vol. 5, No. FEB.
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