Lipoperoxidation affects ochratoxin A biosynthesis in Aspergillus ochraceus and its interaction with wheat seeds

Massimo Reverberi, Federico Punelli, Marzia Scarpari, Emanuela Camera, Slaven Zjalic, Alessandra Ricelli, Corrado Fanelli, Anna Adele Fabbri

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In Aspergillus nidulans, Aspergillus flavus, and Aspergillus parasiticus, lipoperoxidative signalling is crucial for the regulation of mycotoxin biosynthesis, conidiogenesis, and sclerotia formation. Resveratrol, which is a lipoxygenase (LOX) and cyclooxygenase inhibitor, downmodulates the biosynthesis of ochratoxin A (OTA) in Aspergillus ochraceus. In the genome of A. ochraceus, a lox-like sequence (AoloxA; National Center for Biotechnology Information (NCBI) accession number: DQ087531) for a lipoxygenase-like enzyme has been found, which presents high homology (100 identities, 100 positives %, score 555) with a lox gene of Aspergillus fumigatus (NCBI accession number: XM741370). To study how inhibition of oxylipins formation may affect the A. ochraceus metabolism, we have used a ΔAoloxA strain. This mutant displays a different colony morphology, a delayed conidia formation, and a high sclerotia production. When compared to the wild type, the ΔAoloxA strain showed a lower basal activity of LOX and diminished levels of 13-hydroperoxylinoleic acid (HPODE) and other oxylipins derived from linoleic acid. The limited oxylipins formation corresponded to a remarkable inhibition of OTA biosynthesis in the ΔAoloxA strain. Also, wheat seeds (Triticum durum cv Ciccio) inoculated with the ΔAoloxA mutant did not accumulate 9-HPODE, which is a crucial element in the host defence system. Similarly, the expression of the pathogenesis-related protein 1 (PR1) gene in wheat seeds was not enhanced. The results obtained contribute to the current knowledge on the role of lipid peroxidation governed by the AoloxA gene in the morphogenesis, OTA biosynthesis, and in host-pathogen interaction between wheat seeds and A. ochraceus.

Original languageEnglish
Pages (from-to)1935-1946
Number of pages12
JournalApplied Microbiology and Biotechnology
Volume85
Issue number6
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Aspergillus ochraceus
Oxylipins
Triticum
Seeds
Information Centers
Lipoxygenase
Biotechnology
Host-Pathogen Interactions
Aspergillus nidulans
Aspergillus flavus
Lipoxygenase Inhibitors
Aspergillus fumigatus
Fungal Spores
Cyclooxygenase Inhibitors
Mycotoxins
Linoleic Acid
Aspergillus
Morphogenesis
Lipid Peroxidation
Genes

Keywords

  • Lipoperoxidation
  • Lipoxygenase
  • Ochratoxin A
  • Wheat seeds

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Lipoperoxidation affects ochratoxin A biosynthesis in Aspergillus ochraceus and its interaction with wheat seeds. / Reverberi, Massimo; Punelli, Federico; Scarpari, Marzia; Camera, Emanuela; Zjalic, Slaven; Ricelli, Alessandra; Fanelli, Corrado; Fabbri, Anna Adele.

In: Applied Microbiology and Biotechnology, Vol. 85, No. 6, 02.2010, p. 1935-1946.

Research output: Contribution to journalArticle

Reverberi, Massimo ; Punelli, Federico ; Scarpari, Marzia ; Camera, Emanuela ; Zjalic, Slaven ; Ricelli, Alessandra ; Fanelli, Corrado ; Fabbri, Anna Adele. / Lipoperoxidation affects ochratoxin A biosynthesis in Aspergillus ochraceus and its interaction with wheat seeds. In: Applied Microbiology and Biotechnology. 2010 ; Vol. 85, No. 6. pp. 1935-1946.
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AU - Ricelli, Alessandra

AU - Fanelli, Corrado

AU - Fabbri, Anna Adele

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