Lipopolysaccharide modulation of eicosanoid and corticotrophin-releasing hormone release from rat hypothalamic explants and astrocyte cultures in vitro: Evidence for the involvement of prostaglandin E2 but not prostaglandin F(2α) and lack of effect of nerve growth factor

G. Pozzoli, A. Costa, M. Grimaldi, G. Schettini, P. Preziosi, A. Grossman, P. Navarra

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Abstract

Bacterial lipopolysaccharide (LPS) and prostaglandins (PG) E2 and F(2α) are putative activators of the hypothalamo-pituitary-adrenal axis. Certain of the biological effects of LPS may be mediated by cytokines such as interleukin-1β (IL-1β), while IL-1β itself may operate via induction of the prostaglandins and/or nerve growth factor (NGF). As IL-1β stimulates the release of corticotrophin-releasing hormone (CRH) from acute rat hypothalamic explants directly, the effects of these substances on the release of CRH in vitro were investigated in short- and medium-term (20 and 60 min) incubations. The effect of LPS on the release of PGE2 and PGF(2α) from these explants, as well as from cortical astrocyte cultures, was also studied. LPS did not modify the release of CRH, PGE2 or PGF(2α) in 20-min incubations. In 60-min incubations, LPS stimulated the release of PGE2, whereas the release of CRH was weakly, but significantly, reduced; PGF(2α) was not altered. PGE2 significantly stimulated CRH release in the 60-min but not in the 20-min experiments. This effect appeared to be selective for PGE2, since PGF(2α) did not modify CRH release, alone or in combination. LPS also selectively released PGE2 but not PGF(2α) from cortical astrocyte cultures after 24-h incubation. NGF had no effect on the release of explant CRH, regardless of the length of incubation. It was concluded that neither LPS nor NGF acutely stimulate the hypothalamo-pituitary-adrenal axis by a direct action on hypothalamic CRH, while hypothalamic PGE2 may mediate, at least in part, certain of the neuroendocrine responses to LPS.

Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalJournal of Endocrinology
Volume140
Issue number1
Publication statusPublished - 1994

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Eicosanoids
Corticotropin-Releasing Hormone
Prostaglandins F
Nerve Growth Factor
Dinoprostone
Astrocytes
Lipopolysaccharides
Interleukin-1
Pituitary Hormone-Releasing Hormones
In Vitro Techniques
Prostaglandins
Cytokines

ASJC Scopus subject areas

  • Endocrinology

Cite this

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title = "Lipopolysaccharide modulation of eicosanoid and corticotrophin-releasing hormone release from rat hypothalamic explants and astrocyte cultures in vitro: Evidence for the involvement of prostaglandin E2 but not prostaglandin F(2α) and lack of effect of nerve growth factor",
abstract = "Bacterial lipopolysaccharide (LPS) and prostaglandins (PG) E2 and F(2α) are putative activators of the hypothalamo-pituitary-adrenal axis. Certain of the biological effects of LPS may be mediated by cytokines such as interleukin-1β (IL-1β), while IL-1β itself may operate via induction of the prostaglandins and/or nerve growth factor (NGF). As IL-1β stimulates the release of corticotrophin-releasing hormone (CRH) from acute rat hypothalamic explants directly, the effects of these substances on the release of CRH in vitro were investigated in short- and medium-term (20 and 60 min) incubations. The effect of LPS on the release of PGE2 and PGF(2α) from these explants, as well as from cortical astrocyte cultures, was also studied. LPS did not modify the release of CRH, PGE2 or PGF(2α) in 20-min incubations. In 60-min incubations, LPS stimulated the release of PGE2, whereas the release of CRH was weakly, but significantly, reduced; PGF(2α) was not altered. PGE2 significantly stimulated CRH release in the 60-min but not in the 20-min experiments. This effect appeared to be selective for PGE2, since PGF(2α) did not modify CRH release, alone or in combination. LPS also selectively released PGE2 but not PGF(2α) from cortical astrocyte cultures after 24-h incubation. NGF had no effect on the release of explant CRH, regardless of the length of incubation. It was concluded that neither LPS nor NGF acutely stimulate the hypothalamo-pituitary-adrenal axis by a direct action on hypothalamic CRH, while hypothalamic PGE2 may mediate, at least in part, certain of the neuroendocrine responses to LPS.",
author = "G. Pozzoli and A. Costa and M. Grimaldi and G. Schettini and P. Preziosi and A. Grossman and P. Navarra",
year = "1994",
language = "English",
volume = "140",
pages = "103--109",
journal = "Journal of Endocrinology",
issn = "0022-0795",
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TY - JOUR

T1 - Lipopolysaccharide modulation of eicosanoid and corticotrophin-releasing hormone release from rat hypothalamic explants and astrocyte cultures in vitro

T2 - Evidence for the involvement of prostaglandin E2 but not prostaglandin F(2α) and lack of effect of nerve growth factor

AU - Pozzoli, G.

AU - Costa, A.

AU - Grimaldi, M.

AU - Schettini, G.

AU - Preziosi, P.

AU - Grossman, A.

AU - Navarra, P.

PY - 1994

Y1 - 1994

N2 - Bacterial lipopolysaccharide (LPS) and prostaglandins (PG) E2 and F(2α) are putative activators of the hypothalamo-pituitary-adrenal axis. Certain of the biological effects of LPS may be mediated by cytokines such as interleukin-1β (IL-1β), while IL-1β itself may operate via induction of the prostaglandins and/or nerve growth factor (NGF). As IL-1β stimulates the release of corticotrophin-releasing hormone (CRH) from acute rat hypothalamic explants directly, the effects of these substances on the release of CRH in vitro were investigated in short- and medium-term (20 and 60 min) incubations. The effect of LPS on the release of PGE2 and PGF(2α) from these explants, as well as from cortical astrocyte cultures, was also studied. LPS did not modify the release of CRH, PGE2 or PGF(2α) in 20-min incubations. In 60-min incubations, LPS stimulated the release of PGE2, whereas the release of CRH was weakly, but significantly, reduced; PGF(2α) was not altered. PGE2 significantly stimulated CRH release in the 60-min but not in the 20-min experiments. This effect appeared to be selective for PGE2, since PGF(2α) did not modify CRH release, alone or in combination. LPS also selectively released PGE2 but not PGF(2α) from cortical astrocyte cultures after 24-h incubation. NGF had no effect on the release of explant CRH, regardless of the length of incubation. It was concluded that neither LPS nor NGF acutely stimulate the hypothalamo-pituitary-adrenal axis by a direct action on hypothalamic CRH, while hypothalamic PGE2 may mediate, at least in part, certain of the neuroendocrine responses to LPS.

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