Lipopolysaccharide or Whole Bacteria Block the Conversion of Inflammatory Monocytes into Dendritic Cells In Vivo

Gianluca Rotta, Emmerson W. Edwards, Sabina Sangaletti, Clare Bennett, Simona Ronzoni, Mario P. Colombo, Ralph M. Steinman, Gwendalyn J. Randolph, Maria Rescigno

Research output: Contribution to journalArticle

Abstract

Monocytes can develop into dendritic cells (DCs) that migrate to lymph nodes (LNs) and present antigens to T cells. However, we find that this differentiation is blocked when monocytes accumulate subcutaneously in response to bacteria or lipopolysaccharide (LPS). The inhibition of DC differentiation is mediated by the bacteria and in conjunction with inflammatory cells recruited at the site of injection. Inhibition of migratory DC development was reversed in Toll-like receptor (TLR)4-mutated mice when LPS, but not whole bacteria, was injected, suggesting that TLR4 is one but not the only mediator of the inhibition. The block imposed by bacteria was partly relieved by the absence of interleukin (IL)-12 p40, but not by individual absence of several cytokines involved in DC differentiation or in inflammation, i.e., IL-6, IL-10, IL-12 p35, and interferon γ. Consistent with the inability of monocytes to yield migrating DCs, and the finding that other DCs had limited access to particulate or bacterial antigens, these antigens were weakly presented to T cells in the draining LN. These results illustrate that bacteria-associated signals can have a negative regulatory role on adaptive immunity and that local innate responses for containment of infectious bacteria can at least initially supersede development of adaptive responses.

Original languageEnglish
Pages (from-to)1253-1263
Number of pages11
JournalJournal of Experimental Medicine
Volume198
Issue number8
DOIs
Publication statusPublished - Oct 20 2003

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Dendritic Cells
Lipopolysaccharides
Monocytes
Bacteria
Cell Differentiation
Interleukin-12 Subunit p35
Lymph Nodes
Bacterial Antigens
T-Lymphocytes
Antigens
Adaptive Immunity
Interleukin-12
Interleukin-10
Interferons
Interleukin-6
Cytokines
Inflammation
Injections

Keywords

  • Adaptive immunity
  • Inflammation
  • Innate immunity
  • Migration
  • Salmonella typhimurium

ASJC Scopus subject areas

  • Immunology

Cite this

Lipopolysaccharide or Whole Bacteria Block the Conversion of Inflammatory Monocytes into Dendritic Cells In Vivo. / Rotta, Gianluca; Edwards, Emmerson W.; Sangaletti, Sabina; Bennett, Clare; Ronzoni, Simona; Colombo, Mario P.; Steinman, Ralph M.; Randolph, Gwendalyn J.; Rescigno, Maria.

In: Journal of Experimental Medicine, Vol. 198, No. 8, 20.10.2003, p. 1253-1263.

Research output: Contribution to journalArticle

Rotta, Gianluca ; Edwards, Emmerson W. ; Sangaletti, Sabina ; Bennett, Clare ; Ronzoni, Simona ; Colombo, Mario P. ; Steinman, Ralph M. ; Randolph, Gwendalyn J. ; Rescigno, Maria. / Lipopolysaccharide or Whole Bacteria Block the Conversion of Inflammatory Monocytes into Dendritic Cells In Vivo. In: Journal of Experimental Medicine. 2003 ; Vol. 198, No. 8. pp. 1253-1263.
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