Lipoprotein (a) induces angiogenesis on the chick embryo chorioallantoic membrane

D. Ribatti, A. Vacca, F. Giacchetta, S. Cesaretti, M. Anichini, L. Roncali, F. Damacco

Research output: Contribution to journalArticle

Abstract

Background - Both lipoprotein (a) [Lp(a)] and angiogenesis have been shown to be associated with initiation and progression of atherosclerotic plaque. Lp(a) and two neutralizing anti-Lp(a) antibodies were investigated for their capacity to affect the vasoproliferative processes of the chick embryo chorioallantoic membrane (CAM), a useful model for such an investigation. Methods - Gelatin sponges loaded with Lp(a) alone or together with anti-Lp(a) antibodies, or with vehicle alone, phosphate-buffered saline (PBS), were implanted in viva onto the CAM at incubation day 8. Four days later, sponges and the adjacent CAM tissues were assessed for the extent of angiogenesis in terms of microvessel counts. Results - Lp(a)-loaded sponges gave significantly higher counts than those loaded with the Lp(a)-anti-Lp(a) antibodies complex, which overlapped those treated with PBS. The angiogenic response was similar to that obtained with basic fibroblast growth factor, a well-known angiogenic molecule. Conclusion - These data suggest that Lp(a) is capable of inducing angiogenesis in vivo, which might account for its ability to enhance and support atherosclerosis.

Original languageEnglish
Pages (from-to)533-537
Number of pages5
JournalEuropean Journal of Clinical Investigation
Volume28
Issue number7
DOIs
Publication statusPublished - 1998

Fingerprint

Chorioallantoic Membrane
Lipoprotein(a)
Chick Embryo
Membranes
Porifera
Antibodies
Phosphates
Fibroblast Growth Factor 2
Atherosclerotic Plaques
Gelatin
Microvessels
Atherosclerosis
Tissue
Molecules

Keywords

  • Angiogenesis
  • Atherosclerosis
  • Chorioallantoic membrane
  • Lipoprotein(a)

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ribatti, D., Vacca, A., Giacchetta, F., Cesaretti, S., Anichini, M., Roncali, L., & Damacco, F. (1998). Lipoprotein (a) induces angiogenesis on the chick embryo chorioallantoic membrane. European Journal of Clinical Investigation, 28(7), 533-537. https://doi.org/10.1046/j.1365-2362.1998.00322.x

Lipoprotein (a) induces angiogenesis on the chick embryo chorioallantoic membrane. / Ribatti, D.; Vacca, A.; Giacchetta, F.; Cesaretti, S.; Anichini, M.; Roncali, L.; Damacco, F.

In: European Journal of Clinical Investigation, Vol. 28, No. 7, 1998, p. 533-537.

Research output: Contribution to journalArticle

Ribatti, D, Vacca, A, Giacchetta, F, Cesaretti, S, Anichini, M, Roncali, L & Damacco, F 1998, 'Lipoprotein (a) induces angiogenesis on the chick embryo chorioallantoic membrane', European Journal of Clinical Investigation, vol. 28, no. 7, pp. 533-537. https://doi.org/10.1046/j.1365-2362.1998.00322.x
Ribatti, D. ; Vacca, A. ; Giacchetta, F. ; Cesaretti, S. ; Anichini, M. ; Roncali, L. ; Damacco, F. / Lipoprotein (a) induces angiogenesis on the chick embryo chorioallantoic membrane. In: European Journal of Clinical Investigation. 1998 ; Vol. 28, No. 7. pp. 533-537.
@article{69fcefe33a2144018aab8a1d05bf43fe,
title = "Lipoprotein (a) induces angiogenesis on the chick embryo chorioallantoic membrane",
abstract = "Background - Both lipoprotein (a) [Lp(a)] and angiogenesis have been shown to be associated with initiation and progression of atherosclerotic plaque. Lp(a) and two neutralizing anti-Lp(a) antibodies were investigated for their capacity to affect the vasoproliferative processes of the chick embryo chorioallantoic membrane (CAM), a useful model for such an investigation. Methods - Gelatin sponges loaded with Lp(a) alone or together with anti-Lp(a) antibodies, or with vehicle alone, phosphate-buffered saline (PBS), were implanted in viva onto the CAM at incubation day 8. Four days later, sponges and the adjacent CAM tissues were assessed for the extent of angiogenesis in terms of microvessel counts. Results - Lp(a)-loaded sponges gave significantly higher counts than those loaded with the Lp(a)-anti-Lp(a) antibodies complex, which overlapped those treated with PBS. The angiogenic response was similar to that obtained with basic fibroblast growth factor, a well-known angiogenic molecule. Conclusion - These data suggest that Lp(a) is capable of inducing angiogenesis in vivo, which might account for its ability to enhance and support atherosclerosis.",
keywords = "Angiogenesis, Atherosclerosis, Chorioallantoic membrane, Lipoprotein(a)",
author = "D. Ribatti and A. Vacca and F. Giacchetta and S. Cesaretti and M. Anichini and L. Roncali and F. Damacco",
year = "1998",
doi = "10.1046/j.1365-2362.1998.00322.x",
language = "English",
volume = "28",
pages = "533--537",
journal = "European Journal of Clinical Investigation",
issn = "0014-2972",
publisher = "Blackwell Publishing Ltd",
number = "7",

}

TY - JOUR

T1 - Lipoprotein (a) induces angiogenesis on the chick embryo chorioallantoic membrane

AU - Ribatti, D.

AU - Vacca, A.

AU - Giacchetta, F.

AU - Cesaretti, S.

AU - Anichini, M.

AU - Roncali, L.

AU - Damacco, F.

PY - 1998

Y1 - 1998

N2 - Background - Both lipoprotein (a) [Lp(a)] and angiogenesis have been shown to be associated with initiation and progression of atherosclerotic plaque. Lp(a) and two neutralizing anti-Lp(a) antibodies were investigated for their capacity to affect the vasoproliferative processes of the chick embryo chorioallantoic membrane (CAM), a useful model for such an investigation. Methods - Gelatin sponges loaded with Lp(a) alone or together with anti-Lp(a) antibodies, or with vehicle alone, phosphate-buffered saline (PBS), were implanted in viva onto the CAM at incubation day 8. Four days later, sponges and the adjacent CAM tissues were assessed for the extent of angiogenesis in terms of microvessel counts. Results - Lp(a)-loaded sponges gave significantly higher counts than those loaded with the Lp(a)-anti-Lp(a) antibodies complex, which overlapped those treated with PBS. The angiogenic response was similar to that obtained with basic fibroblast growth factor, a well-known angiogenic molecule. Conclusion - These data suggest that Lp(a) is capable of inducing angiogenesis in vivo, which might account for its ability to enhance and support atherosclerosis.

AB - Background - Both lipoprotein (a) [Lp(a)] and angiogenesis have been shown to be associated with initiation and progression of atherosclerotic plaque. Lp(a) and two neutralizing anti-Lp(a) antibodies were investigated for their capacity to affect the vasoproliferative processes of the chick embryo chorioallantoic membrane (CAM), a useful model for such an investigation. Methods - Gelatin sponges loaded with Lp(a) alone or together with anti-Lp(a) antibodies, or with vehicle alone, phosphate-buffered saline (PBS), were implanted in viva onto the CAM at incubation day 8. Four days later, sponges and the adjacent CAM tissues were assessed for the extent of angiogenesis in terms of microvessel counts. Results - Lp(a)-loaded sponges gave significantly higher counts than those loaded with the Lp(a)-anti-Lp(a) antibodies complex, which overlapped those treated with PBS. The angiogenic response was similar to that obtained with basic fibroblast growth factor, a well-known angiogenic molecule. Conclusion - These data suggest that Lp(a) is capable of inducing angiogenesis in vivo, which might account for its ability to enhance and support atherosclerosis.

KW - Angiogenesis

KW - Atherosclerosis

KW - Chorioallantoic membrane

KW - Lipoprotein(a)

UR - http://www.scopus.com/inward/record.url?scp=0031857136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031857136&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2362.1998.00322.x

DO - 10.1046/j.1365-2362.1998.00322.x

M3 - Article

C2 - 9726032

AN - SCOPUS:0031857136

VL - 28

SP - 533

EP - 537

JO - European Journal of Clinical Investigation

JF - European Journal of Clinical Investigation

SN - 0014-2972

IS - 7

ER -