Lipoprotein(a) and proliferative diabetic retinopathy in Type II diabetes mellitus: A role for isoforms with low molecular weight

P. Buscaglia, C. Gazzaruso, A. Garzaniti, G. Bonetti, A. Porta, A. Negri, G. Vandelli, G. Finardi, P. Fratino, D. Geroldi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Apolipoprotein(a) [apo(a)] displays a remarkable genetic heterogeneity of which the physiopathologic mechanisms are yet unknown. Its homology with plasminogen also suggests a pro-thrombotic role. To investigate whether or not lipoprotein(a) [Lp(a)] concentration and/or apo(a) isoforms correlate with the severity of diabetic retinopathy (DR), 113 Type II diabetic patients were studied. By fluorescein angiography, 3 classes of DR were considered: 'null' (40 subjects), 'non-proliferative' (37 subjects) and 'proliferative' (36 subjects). Apo(a) isoforms were detected by a capillary Western Blotting technique, resulting in the identification of 21 apo(a) isoforms with molecular weight (MW) varying from 400 to 775 kDa. Lp(a) plasma concentration did not correlate with the degree of DR (15.6 mg/dl in 'null', 17.8 mg/dl in 'non-proliferative' and 20.6 mg/dl in 'proliferative' DR group, p = 0.399). On the contrary, in subjects with proliferative DR there was a significant prevalence (65%) of low MW apo(a) isoforms (cut-off between 640 and 655 kDa), as compared to null DR (26%, p <0.01) and to non-proliferative DR (35%, p <0.05). In conclusion, low MW apo(a) isoforms could represent another possible biological marker of the genetic risk for the development and progression of retinal diabetic microangiopathy.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalDiabetes, Nutrition and Metabolism - Clinical and Experimental
Volume9
Issue number3
Publication statusPublished - 1996

Fingerprint

diabetic retinopathy
Lipoprotein(a)
Diabetic Retinopathy
noninsulin-dependent diabetes mellitus
lipoproteins
Apoprotein(a)
Type 2 Diabetes Mellitus
apolipoproteins
Protein Isoforms
Molecular Weight
molecular weight
microangiopathy
plasminogen
Diabetic Angiopathies
Genetic Heterogeneity
Fluorescein Angiography
Plasminogen
fluorescein
biomarkers
Western blotting

Keywords

  • Apo(a) isoforms
  • Diabetic retinopathy
  • Lipoprotein(a)
  • Microangiopathy

ASJC Scopus subject areas

  • Food Science
  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Lipoprotein(a) and proliferative diabetic retinopathy in Type II diabetes mellitus : A role for isoforms with low molecular weight. / Buscaglia, P.; Gazzaruso, C.; Garzaniti, A.; Bonetti, G.; Porta, A.; Negri, A.; Vandelli, G.; Finardi, G.; Fratino, P.; Geroldi, D.

In: Diabetes, Nutrition and Metabolism - Clinical and Experimental, Vol. 9, No. 3, 1996, p. 129-137.

Research output: Contribution to journalArticle

Buscaglia, P, Gazzaruso, C, Garzaniti, A, Bonetti, G, Porta, A, Negri, A, Vandelli, G, Finardi, G, Fratino, P & Geroldi, D 1996, 'Lipoprotein(a) and proliferative diabetic retinopathy in Type II diabetes mellitus: A role for isoforms with low molecular weight', Diabetes, Nutrition and Metabolism - Clinical and Experimental, vol. 9, no. 3, pp. 129-137.
Buscaglia, P. ; Gazzaruso, C. ; Garzaniti, A. ; Bonetti, G. ; Porta, A. ; Negri, A. ; Vandelli, G. ; Finardi, G. ; Fratino, P. ; Geroldi, D. / Lipoprotein(a) and proliferative diabetic retinopathy in Type II diabetes mellitus : A role for isoforms with low molecular weight. In: Diabetes, Nutrition and Metabolism - Clinical and Experimental. 1996 ; Vol. 9, No. 3. pp. 129-137.
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AU - Garzaniti, A.

AU - Bonetti, G.

AU - Porta, A.

AU - Negri, A.

AU - Vandelli, G.

AU - Finardi, G.

AU - Fratino, P.

AU - Geroldi, D.

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AB - Apolipoprotein(a) [apo(a)] displays a remarkable genetic heterogeneity of which the physiopathologic mechanisms are yet unknown. Its homology with plasminogen also suggests a pro-thrombotic role. To investigate whether or not lipoprotein(a) [Lp(a)] concentration and/or apo(a) isoforms correlate with the severity of diabetic retinopathy (DR), 113 Type II diabetic patients were studied. By fluorescein angiography, 3 classes of DR were considered: 'null' (40 subjects), 'non-proliferative' (37 subjects) and 'proliferative' (36 subjects). Apo(a) isoforms were detected by a capillary Western Blotting technique, resulting in the identification of 21 apo(a) isoforms with molecular weight (MW) varying from 400 to 775 kDa. Lp(a) plasma concentration did not correlate with the degree of DR (15.6 mg/dl in 'null', 17.8 mg/dl in 'non-proliferative' and 20.6 mg/dl in 'proliferative' DR group, p = 0.399). On the contrary, in subjects with proliferative DR there was a significant prevalence (65%) of low MW apo(a) isoforms (cut-off between 640 and 655 kDa), as compared to null DR (26%, p <0.01) and to non-proliferative DR (35%, p <0.05). In conclusion, low MW apo(a) isoforms could represent another possible biological marker of the genetic risk for the development and progression of retinal diabetic microangiopathy.

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