TY - JOUR
T1 - Liposomes as a Putative Tool to Investigate NAADP Signaling in Vasculogenesis
AU - Di Nezza, Francesca
AU - Zuccolo, Estella
AU - Poletto, Valentina
AU - Rosti, Vittorio
AU - De Luca, Antonio
AU - Moccia, Francesco
AU - Guerra, Germano
AU - Ambrosone, Luigi
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Nicotinic acid adenine dinucleotide phosphate (NAADP) is the newest discovered intracellular second messengers, which is able to release Ca2+ stored within endolysosomal (EL) vesicles. NAADP-induced Ca2+ signals mediate a growing number of cellular functions, ranging from proliferation to muscle contraction and differentiation. Recently, NAADP has recently been shown to regulate angiogenesis by promoting endothelial cell growth. It is, however, still unknown whether NAADP stimulates proliferation also in endothelial progenitor cells, which are mobilized in circulation after an ischemic insult to induce tissue revascularization. Herein, we described a novel approach to prepare NAADP-containing liposomes, which are highly cell membrane permeable and are therefore amenable for stimulating cell activity. Accordingly, NAADP-containing liposomes evoked an increase in intracellular Ca2+ concentration, which was inhibited by NED-19, a selective inhibitor of NAADP-induced Ca2+ release. Furthermore, NAADP-containing liposomes promoted EPC proliferation, a process which was inhibited by NED-19 and BAPTA, a membrane permeable intracellular Ca2+ buffer. Therefore, NAADP-containing liposomes stand out as a promising tool to promote revascularization of hypoxic/ischemic tissues by favoring EPC proliferation. J. Cell. Biochem. 118: 3722–3729, 2017.
AB - Nicotinic acid adenine dinucleotide phosphate (NAADP) is the newest discovered intracellular second messengers, which is able to release Ca2+ stored within endolysosomal (EL) vesicles. NAADP-induced Ca2+ signals mediate a growing number of cellular functions, ranging from proliferation to muscle contraction and differentiation. Recently, NAADP has recently been shown to regulate angiogenesis by promoting endothelial cell growth. It is, however, still unknown whether NAADP stimulates proliferation also in endothelial progenitor cells, which are mobilized in circulation after an ischemic insult to induce tissue revascularization. Herein, we described a novel approach to prepare NAADP-containing liposomes, which are highly cell membrane permeable and are therefore amenable for stimulating cell activity. Accordingly, NAADP-containing liposomes evoked an increase in intracellular Ca2+ concentration, which was inhibited by NED-19, a selective inhibitor of NAADP-induced Ca2+ release. Furthermore, NAADP-containing liposomes promoted EPC proliferation, a process which was inhibited by NED-19 and BAPTA, a membrane permeable intracellular Ca2+ buffer. Therefore, NAADP-containing liposomes stand out as a promising tool to promote revascularization of hypoxic/ischemic tissues by favoring EPC proliferation. J. Cell. Biochem. 118: 3722–3729, 2017.
KW - Ca SIGNALING
KW - ENDOTHELIAL PROGENITOR CELLS
KW - LIPOSOMES
KW - NAADP
KW - VASCULOGENESIS
UR - http://www.scopus.com/inward/record.url?scp=85019616646&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019616646&partnerID=8YFLogxK
U2 - 10.1002/jcb.26019
DO - 10.1002/jcb.26019
M3 - Article
C2 - 28374913
AN - SCOPUS:85019616646
VL - 118
SP - 3722
EP - 3729
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
SN - 0730-2312
IS - 11
ER -