Lipoxygenase Products Induce Ultraweak Light Emission from Human Erythroleukemia Cells

Mauro Maccarrone, Nicola Rosato, Alessandro Finazzi Agrò

Research output: Contribution to journalArticlepeer-review

Abstract

Several lipoxygenase products were able to enhance ultraweak light emission and membrane permeability of human erythroleukemia K562 cells. In particular, 13-hydroperoxyoctadecadienoic acid (13-HPOD) was more effective than hydrophilic hydroperoxides, like H2O2 and fert-butyl hydroperoxide. The enhancement of luminescence induced by 13-HPOD was inhibited by superoxide and hydroxyl radical scavengers. The effect of 13-HPOD was inhibited by superoxide and hydroxyl radical scavengers. The effect of 13-HPOD was potentiated by the calcium ionophore A23187 and inhibited by the calcium chelator EDTA, and was observed also in liposomes containing unsaturated lipids. Cholesterol enrichment, which decreases the membrane fluidity, did not modify the effect of 13-HPOD on K562 cells.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalLuminescence
Volume12
Issue number6
Publication statusPublished - Nov 1997

Keywords

  • Antioxidants
  • Calcium
  • Hydroperoxides
  • Liposomes
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)

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