Liquid biopsy in the diagnosis of HPV DNA in breast lesions

Sabrina De Carolis; Alice Pellegrini; Donatella Santini; Claudio Ceccarelli; Antonio De Leo; Federica Alessandrini; Chiara Arienti; Sara Pignatta; Anna Tesei; Vilma Mantovani; Claudio Zamagni; Mario Taffurelli; Pasquale Sansone; Massimiliano Bonafé; Monica Cricca

doi:10.2217/fmb-2017-0145

Liquid biopsy in the diagnosis of HPV DNA in breast lesions

Sabrina De Carolis, Alice Pellegrini, Donatella Santini, Claudio Ceccarelli, Antonio De Leo, Federica Alessandrini, Chiara Arienti, Sara Pignatta, Anna Tesei, Vilma Mantovani, Claudio Zamagni, Mario Taffurelli, Pasquale Sansone, Massimiliano Bonafé, Monica Cricca

Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

AIM: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens.

MATERIALS & METHODS: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types.

RESULTS: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas. We also show the presence of HPV DNA in the serum-derived extracellular vesicles.

CONCLUSION: Our data supports the use of liquid biopsy to detect HPV DNA in breast pathology.

Original language	English
Pages (from-to)	187-194
Number of pages	8
Journal	Future Microbiology
Volume	13
DOIs	https://doi.org/10.2217/fmb-2017-0145
Publication status	Published - Feb 2018

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Liquid biopsy in the diagnosis of HPV DNA in breast lesions. / Carolis, Sabrina De; Pellegrini, Alice; Santini, Donatella; Ceccarelli, Claudio; De Leo, Antonio; Alessandrini, Federica; Arienti, Chiara ; Pignatta, Sara ; Tesei, Anna; Mantovani, Vilma; Zamagni, Claudio; Taffurelli, Mario; Sansone, Pasquale; Bonafé, Massimiliano; Cricca, Monica.

In: Future Microbiology, Vol. 13, 02.2018, p. 187-194.

Research output: Contribution to journal › Article › peer-review

Carolis, Sabrina De ; Pellegrini, Alice ; Santini, Donatella ; Ceccarelli, Claudio ; De Leo, Antonio ; Alessandrini, Federica ; Arienti, Chiara ; Pignatta, Sara ; Tesei, Anna ; Mantovani, Vilma ; Zamagni, Claudio ; Taffurelli, Mario ; Sansone, Pasquale ; Bonafé, Massimiliano ; Cricca, Monica. / Liquid biopsy in the diagnosis of HPV DNA in breast lesions. In: Future Microbiology. 2018 ; Vol. 13. pp. 187-194.

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abstract = "AIM: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens.MATERIALS & METHODS: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types.RESULTS: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas. We also show the presence of HPV DNA in the serum-derived extracellular vesicles.CONCLUSION: Our data supports the use of liquid biopsy to detect HPV DNA in breast pathology.",

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AU - Carolis, Sabrina De

AU - Pellegrini, Alice

AU - Santini, Donatella

AU - Ceccarelli, Claudio

AU - De Leo, Antonio

AU - Alessandrini, Federica

AU - Arienti, Chiara

AU - Pignatta, Sara

AU - Tesei, Anna

AU - Mantovani, Vilma

AU - Zamagni, Claudio

AU - Taffurelli, Mario

AU - Sansone, Pasquale

AU - Bonafé, Massimiliano

AU - Cricca, Monica

PY - 2018/2

Y1 - 2018/2

N2 - AIM: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens.MATERIALS & METHODS: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types.RESULTS: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas. We also show the presence of HPV DNA in the serum-derived extracellular vesicles.CONCLUSION: Our data supports the use of liquid biopsy to detect HPV DNA in breast pathology.

AB - AIM: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens.MATERIALS & METHODS: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types.RESULTS: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas. We also show the presence of HPV DNA in the serum-derived extracellular vesicles.CONCLUSION: Our data supports the use of liquid biopsy to detect HPV DNA in breast pathology.

KW - Journal Article

U2 - 10.2217/fmb-2017-0145

DO - 10.2217/fmb-2017-0145

M3 - Article

C2 - 28975808

VL - 13

SP - 187

EP - 194

JO - Future Microbiology

JF - Future Microbiology

SN - 1746-0913

ER -

Liquid biopsy in the diagnosis of HPV DNA in breast lesions

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