Liquid chromatographic determination of free serotonin in human plasma

Bianca Marasini, Maria Luisa Biondi, Angelo Agostoni

Research output: Contribution to journalArticle

Abstract

The complex background of interfering substances makes plasma pretreatment necessary before performing liquid-chromatographic quantitation of circulating serotonin. We compared two isolation procedures (extraction into butanol and isolation on a weak cation-exchange resin) before chromatography for measuring free serotonin from human plasma. Sensitivity, specificity and recovery were all evaluated. The cation-exchange resin yielded chromatograms with no interfering peaks, was cheaper and took less processing time than butanol extraction. We conclude that the clean-up of human plasma by a cation-exchange resin is suitable and accurate for routine liquid-chromatographic determination of circulating serotonin.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalLa Ricerca in Clinica e in Laboratorio
Volume17
Issue number2
DOIs
Publication statusPublished - Apr 1987

Keywords

  • Butanol
  • Cation-exchange resin
  • High-performance liquid chromatography
  • Human plasma
  • Serotonin

ASJC Scopus subject areas

  • Clinical Biochemistry

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