Liquid chromatography tandem mass spectrometry assay for fast and sensitive quantification of estrone-sulfate

Giuseppe Corona, Caterina Elia, Bruno Casetta, Alessandro Da Ponte, Lino Del Pup, Enzo Ottavian, Giuseppe Toffoli

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: The circulating pool of estrone-sulfate is considered as a "reservoir" of slowly-metabolized estrogen that can be exploited for assessing overall individual estrogenicity. The aim of this study was to develop a rapid and sensitive liquid chromatography-tandem mass spectrometry assay for the determination of estrone-sulfate, suitable for routine clinical investigations. Methods: The proposed assay is based on a simple protein precipitation procedure and on a fast measurement with a triple-quadrupole mass spectrometer operating in negative ion mode and in multiple reaction monitoring. The method was assessed for intra- and inter-day precision, accuracy, recovery, and clinical suitability. A comparison with available radioimmunoassay was also performed. Results: The LC-MS/MS method is able to detect estrone-sulfate concentrations ≤1 pg/mL and has a low limit of quantification of 7.8 pg/mL. Intra- and inter-day precision and accuracy were less than 10.5% and 5.0% respectively. The recovery was in the range of 93%-110%. When compared with radioimmunoassay the method resulted more accurate and therefore more suitable for quantifying the estrone-sulfate in different clinical settings, including patients treated with aromatase inhibitors. Conclusions: The proposed LC-MS/MS method represents a convincing alternative to the immunoassay for a fast, cost-effective and reliable measurement of estrone-sulfate in routine clinical investigations and in large epidemiological studies. It may contribute in shedding a new light on the diagnostic value of estrone-sulfate in normal and pathological conditions.

Original languageEnglish
Pages (from-to)574-580
Number of pages7
JournalClinica Chimica Acta
Volume411
Issue number7-8
DOIs
Publication statusPublished - Apr 2010

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Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Assays
Radioimmunoassay
Recovery
Aromatase Inhibitors
Mass spectrometers
Immunoassay
estrone sulfate
Epidemiologic Studies
Estrogens
Negative ions
Ions
Light
Costs and Cost Analysis
Monitoring
Costs
Proteins

Keywords

  • Aromatase inhibitors
  • Estrogen
  • Estrogen suppression
  • Estrone-sulfate
  • LC-MS/MS
  • Menopausal

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Liquid chromatography tandem mass spectrometry assay for fast and sensitive quantification of estrone-sulfate. / Corona, Giuseppe; Elia, Caterina; Casetta, Bruno; Da Ponte, Alessandro; Del Pup, Lino; Ottavian, Enzo; Toffoli, Giuseppe.

In: Clinica Chimica Acta, Vol. 411, No. 7-8, 04.2010, p. 574-580.

Research output: Contribution to journalArticle

Corona, Giuseppe ; Elia, Caterina ; Casetta, Bruno ; Da Ponte, Alessandro ; Del Pup, Lino ; Ottavian, Enzo ; Toffoli, Giuseppe. / Liquid chromatography tandem mass spectrometry assay for fast and sensitive quantification of estrone-sulfate. In: Clinica Chimica Acta. 2010 ; Vol. 411, No. 7-8. pp. 574-580.
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