Live-cell 3D super-resolution imaging in thick biological samples

Francesca Cella Zanacchi; Zeno Lavagnino; Michela Perrone Donnorso; Alessio Del Bue; Laura Furia; Mario Faretta; Alberto Diaspro

doi:10.1038/nmeth.1744

Live-cell 3D super-resolution imaging in thick biological samples

Francesca Cella Zanacchi, Zeno Lavagnino, Michela Perrone Donnorso, Alessio Del Bue, Laura Furia, Mario Faretta, Alberto Diaspro

Istituto Europeo di Oncologia

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.

Original language	English
Pages (from-to)	1047-1050
Number of pages	4
Journal	Nature Methods
Volume	8
Issue number	12
DOIs	https://doi.org/10.1038/nmeth.1744
Publication status	Published - Dec 2011

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Biochemistry
Cell Biology

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10.1038/nmeth.1744

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abstract = "We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.",

author = "{Cella Zanacchi}, Francesca and Zeno Lavagnino and {Perrone Donnorso}, Michela and {Del Bue}, Alessio and Laura Furia and Mario Faretta and Alberto Diaspro",

year = "2011",

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AU - Cella Zanacchi, Francesca

AU - Lavagnino, Zeno

AU - Perrone Donnorso, Michela

AU - Del Bue, Alessio

AU - Furia, Laura

AU - Faretta, Mario

AU - Diaspro, Alberto

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Live-cell 3D super-resolution imaging in thick biological samples

Abstract

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