Abstract
We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.
Original language | English |
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Pages (from-to) | 1047-1050 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 8 |
Issue number | 12 |
DOIs | |
Publication status | Published - Dec 2011 |
ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Biochemistry
- Cell Biology