Live-cell 3D super-resolution imaging in thick biological samples

Francesca Cella Zanacchi, Zeno Lavagnino, Michela Perrone Donnorso, Alessio Del Bue, Laura Furia, Mario Faretta, Alberto Diaspro

Research output: Contribution to journalArticle

251 Citations (Scopus)

Abstract

We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.

Original languageEnglish
Pages (from-to)1047-1050
Number of pages4
JournalNature Methods
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 2011

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Lighting
Cellular Spheroids
Imaging techniques
Molecules
Signal-To-Noise Ratio
Microscopy
Signal to noise ratio
Microscopic examination
Scattering

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Cella Zanacchi, F., Lavagnino, Z., Perrone Donnorso, M., Del Bue, A., Furia, L., Faretta, M., & Diaspro, A. (2011). Live-cell 3D super-resolution imaging in thick biological samples. Nature Methods, 8(12), 1047-1050. https://doi.org/10.1038/nmeth.1744

Live-cell 3D super-resolution imaging in thick biological samples. / Cella Zanacchi, Francesca; Lavagnino, Zeno; Perrone Donnorso, Michela; Del Bue, Alessio; Furia, Laura; Faretta, Mario; Diaspro, Alberto.

In: Nature Methods, Vol. 8, No. 12, 12.2011, p. 1047-1050.

Research output: Contribution to journalArticle

Cella Zanacchi, F, Lavagnino, Z, Perrone Donnorso, M, Del Bue, A, Furia, L, Faretta, M & Diaspro, A 2011, 'Live-cell 3D super-resolution imaging in thick biological samples', Nature Methods, vol. 8, no. 12, pp. 1047-1050. https://doi.org/10.1038/nmeth.1744
Cella Zanacchi F, Lavagnino Z, Perrone Donnorso M, Del Bue A, Furia L, Faretta M et al. Live-cell 3D super-resolution imaging in thick biological samples. Nature Methods. 2011 Dec;8(12):1047-1050. https://doi.org/10.1038/nmeth.1744
Cella Zanacchi, Francesca ; Lavagnino, Zeno ; Perrone Donnorso, Michela ; Del Bue, Alessio ; Furia, Laura ; Faretta, Mario ; Diaspro, Alberto. / Live-cell 3D super-resolution imaging in thick biological samples. In: Nature Methods. 2011 ; Vol. 8, No. 12. pp. 1047-1050.
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