Live-cell 3D super-resolution imaging in thick biological samples

Francesca Cella Zanacchi, Zeno Lavagnino, Michela Perrone Donnorso, Alessio Del Bue, Laura Furia, Mario Faretta, Alberto Diaspro

Research output: Contribution to journalArticlepeer-review


We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.

Original languageEnglish
Pages (from-to)1047-1050
Number of pages4
JournalNature Methods
Issue number12
Publication statusPublished - Dec 2011

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology


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