Live Monitoring of Blastemal Cell Contributions during Appendage Regeneration

Valerie A. Tornini, Alberto Puliafito, Leslie A. Slota, John D. Thompson, Gregory Nachtrab, Anna Lila Kaushik, Marika Kapsimali, Luca Primo, Stefano Di Talia, Kenneth D. Poss

Research output: Contribution to journalArticle

Abstract

The blastema is a mass of progenitor cells that enables regeneration of amputated salamander limbs or fish fins. Methodology to label and track blastemal cell progeny has been deficient, restricting our understanding of appendage regeneration. Here, we created a system for clonal analysis and quantitative imaging of hundreds of blastemal cells and their respective progeny in living adult zebrafish undergoing fin regeneration. Amputation stimulates resident cells within a limited recruitment zone to reset proximodistal (PD) positional information and assemble the blastema. Within the newly formed blastema, the spatial coordinates of connective tissue progenitors are predictive of their ultimate contributions to regenerated skeletal structures, indicating early development of an approximate PD pre-pattern. Calcineurin regulates size recovery by controlling the average number of progeny divisions without disrupting this pre-pattern. Our longitudinal clonal analyses of regenerating zebrafish fins provide evidence that connective tissue progenitors are rapidly organized into a scalable blueprint of lost structures.

Original languageEnglish
Pages (from-to)2981-2991
Number of pages11
JournalCurrent Biology
Volume26
Issue number22
DOIs
Publication statusPublished - Nov 21 2016

Fingerprint

appendages
fins
Regeneration
Zebrafish
Tissue
Danio rerio
Blueprints
connective tissues
Connective Tissue
Monitoring
monitoring
Calcineurin
Fish
Labels
Urodela
amputation
cells
limbs (animal)
Amputation
salamanders and newts

Keywords

  • appendage
  • blastema
  • clonal analysis
  • fibroblast
  • fin
  • positional identity
  • regeneration
  • tph1b
  • vertebrate
  • zebrafish

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Tornini, V. A., Puliafito, A., Slota, L. A., Thompson, J. D., Nachtrab, G., Kaushik, A. L., ... Poss, K. D. (2016). Live Monitoring of Blastemal Cell Contributions during Appendage Regeneration. Current Biology, 26(22), 2981-2991. https://doi.org/10.1016/j.cub.2016.08.072

Live Monitoring of Blastemal Cell Contributions during Appendage Regeneration. / Tornini, Valerie A.; Puliafito, Alberto; Slota, Leslie A.; Thompson, John D.; Nachtrab, Gregory; Kaushik, Anna Lila; Kapsimali, Marika; Primo, Luca; Di Talia, Stefano; Poss, Kenneth D.

In: Current Biology, Vol. 26, No. 22, 21.11.2016, p. 2981-2991.

Research output: Contribution to journalArticle

Tornini, VA, Puliafito, A, Slota, LA, Thompson, JD, Nachtrab, G, Kaushik, AL, Kapsimali, M, Primo, L, Di Talia, S & Poss, KD 2016, 'Live Monitoring of Blastemal Cell Contributions during Appendage Regeneration', Current Biology, vol. 26, no. 22, pp. 2981-2991. https://doi.org/10.1016/j.cub.2016.08.072
Tornini VA, Puliafito A, Slota LA, Thompson JD, Nachtrab G, Kaushik AL et al. Live Monitoring of Blastemal Cell Contributions during Appendage Regeneration. Current Biology. 2016 Nov 21;26(22):2981-2991. https://doi.org/10.1016/j.cub.2016.08.072
Tornini, Valerie A. ; Puliafito, Alberto ; Slota, Leslie A. ; Thompson, John D. ; Nachtrab, Gregory ; Kaushik, Anna Lila ; Kapsimali, Marika ; Primo, Luca ; Di Talia, Stefano ; Poss, Kenneth D. / Live Monitoring of Blastemal Cell Contributions during Appendage Regeneration. In: Current Biology. 2016 ; Vol. 26, No. 22. pp. 2981-2991.
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