Liver fatty acid-binding protein in two cases of human lipid storage

Lodovica Vergani, Marina Fanin, Andrea Martinuzzi, Andrea Galassi, Andrea Appi, Rosalba Carrozzo, Maurizio Rosa, Corrado Angelini

Research output: Contribution to journalArticlepeer-review

Abstract

FABPs in the various tissues play an important role in the intracellular fatty acid transport and metabolism. Reye's syndrome (RS) and multisystemic lipid storage (MLS) are human disorders characterized by a disturbance of lipid metabolism of unknown etiology. We investigated for the first time L-FABP in these two conditions. Affinity purified antibodies against chicken L-FABP were raised in rabbits, and found to cross-react specifically with partially purified human L-FABP. L-FABP content in liver samples of two patients with RS and MLS was investigated by immuno-histochemistry, SDS-PAGE and ELISA. L-FABP immuno-histochemistry showed increased reactivity in the liver of RS patient and normal reactivity in MLS liver. L-FABP increase in RS liver was confirmed by densitometry of SDS-PAGE and ELISA method. By these two methods the increase amounted to 180% and 199% (p <0.02), respectively, as compared to controls. A possible role of L-FABP in the pathogenesis of RS is discussed.

Original languageEnglish
Pages (from-to)225-230
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume98
Issue number1-2
DOIs
Publication statusPublished - Oct 1990

Keywords

  • FABP
  • fatty liver
  • L-FABP
  • multisystemic lipid storage
  • Reye's syndrome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

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