The liver protective effects of silymarin have been investigated in isolated hepatocytes suspended in vitro in sucrose solutions or graded hypotonicity. Cells preincubated with the drug at the concentration of 10-4M were found to be entirely resistant or relatively little affected when exposed to hypotonic solutions which have a lethal effect on a considerable proportion of the control liver cells, as shown by the determination in the cell preparations (1) of the proportion trypan blue positive (broken) cells, (2) of the protein synthesizing capacity. The protection was reduced but not abolished upon removal of the drug and cell washing. A smaller effect was observed with a drug concentration of 10-5M. At 10-6M the effect was no longer detectable. Chlorpromazine, a drug which belongs to the group of the so called 'unspecific membrane stabilizers', was without effect in this test.
|Number of pages||5|
|Publication status||Published - 1976|
ASJC Scopus subject areas
- Organic Chemistry
- Drug Discovery