Liver X receptor agonist treatment regulates inflammatory response after spinal cord trauma

Irene Paterniti, Tiziana Genovese, Emanuela Mazzon, Concetta Crisafulli, Rosanna Di Paola, Maria Galuppo, Placido Bramanti, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

Abstract

Liver X receptor α (LXRα) and LXRβ are members of the nuclear receptor superfamily of ligand-activated transcription factors. The aim of this study was to investigate the effects of T0901317, a potent LXR receptor ligand, in a mouse model of spinal cord injury (SCI). SCI was induced by the application of vascular clips (force of 24 g) to the dura via a four-level T5-T8 laminectomy in mice. Treatment with T0901317, 1 and 6 h after the SCI, significantly decreased (i) the degree of spinal cord inflammation and tissue injury (histological score); (ii) neutrophil infiltration (myeloperoxidase activity); (iii) inducible nitric oxide synthase expression; (iv) nitrotyrosine, lipid peroxidation, and poly-ADP-ribose formation; (v) pro-inflammatory cytokines expression; (vi) nuclear factor-kappa B activation; and (vii) apoptosis (terminal deoxynucleotidyltransferase-mediated UTP end labeling staining, FAS ligand, Bax, and Bcl-2 expression). Moreover, T0901317 significantly ameliorated the loss of limb function (evaluated by motor recovery score). These data suggest that LXR ligand may be useful in the treatment of inflammation associated with SCI.

Original languageEnglish
Pages (from-to)611-624
Number of pages14
JournalJournal of Neurochemistry
Volume112
Issue number3
DOIs
Publication statusPublished - Feb 2010

Keywords

  • Apoptosis
  • Cytokines
  • Nuclear factor-kappa B
  • Spinal cord injury
  • T0901317

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Liver X receptor agonist treatment regulates inflammatory response after spinal cord trauma'. Together they form a unique fingerprint.

  • Cite this