LNCIB human full-length cDNAs collection: Towards a better comprehension of the human transcriptome

Emiliano Dalla, Roberto Verardo, Dejan Lazarević, Luigi Marchionni, James F. Reid, Nabil Bahar, Enio Klarić, Giacomo Marcuzzi, Riccardo Marzio, Anna Belgrano, Danilo Licastro, Claudio Schneider

Research output: Contribution to journalArticlepeer-review

Abstract

LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23 000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

Original languageEnglish
Pages (from-to)967-970
Number of pages4
JournalComptes Rendus - Biologies
Volume326
Issue number10-11
DOIs
Publication statusPublished - Oct 2003

Keywords

  • Full-length cDNA
  • Functional analysis
  • Human transcriptome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Developmental Biology

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